Lurbinah Week 6

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Lizzy Urbina

Template:lurbinah

Assignments


Weekly Assigments

Class Journal Assigments

Purpose

The purpose of this assigment is to answer and analyze the question proposed on preovoius week using data provided by Markham etl.

Question

Are the interim visits important to record and analyze the decline of CD4 T cells in order to accurately map the progression of HIV evolution?

Methods/Results

Statistical Analysis of CD4 T-cells count and sequenced data.

  • We analyzed the data collected from Markham et al. 1998 data table, and gather the information needed to make our own table and graph
  • The data used for this porject was the first and last CD4 T cell count with its corresponding sequence
    • Some subjects had more visits but not record of DNA being sequenced, these values of CD4 T cells counts were not used in the analysis.
  • Table used to graph and record data. The highligted subjects had more CD4 T cells counts record that were not used, due to the lack of DNA sequence.

Subjectvisitdata.png

Graph and calculate the slope in order to classify the subjects.

  • Based on each of the data collected for each subject, a graph was elaborated to calculate the rate (slope) of change of the CD4 T cell counts.
  • The scale used was the same in all the graphs.
  • Example of Graphs

Subject8graph.png Subject4graph.png Subject6graph.png

Classidication of Subjects

  • Based on the rate of change the subjects were classified into Non-Progressor, Moderate Progressors and Rapid Progressors.
  • Rules for Classification
    • Subjects with slope greater than -100 were classified as non-progressors.
    • Subjects with slope from -100 to -300 were classified as moderate progressors.
    • Subjects with slopes with a minimun of -350 were classified as rapid Progressors.
  • Tables with the Classification

Non Progressors Moderate Progressors Rapid Progressors

Compare and contrast our classification and Markham’s.

  • The only subjects with a discrepancy from Marks classification are 5, 6, 7
    • Subject 5,6,7 were classified as moderate progressor and in our analysis 5 and 6 fall under non Progressor and and 7 as rapid progressor.
      • This could be due to the CD4 T cell count values that were not taken into consideration.

Creation of the phylogenetic trees of each subject.

  • Phylogenetic tree where elobarated for each subject using their first and last visit sequences to look for similarities and differences among and within each group.

Treesubject2.png Treesubject6.png Treesubject12.png Treesubject5.png Treesubject13.png Treesubject8.png Treesubject9.png Treesubject14.png Treesubject4.png Treesubject1.png Treesubject7.png Treesubject10.png Treesubject3.png Treesubject11.png Treesubject15.png

Data and Files

Conclusions

CD4 T-cell decline is directly proportional to the evolution of HIV. Additonally each group responded differently to the ability or selection to change. Non-Progressors favored absence of change, maintaining stable CD4 T-cell counts.This was concluded from the trend showed in the phylogetic trees; Non-Progressors showed less divergence and diversity. However, based on the increase in diversity and divergence in the phylogenect trees moderate and Rapid Progressors showed; progressors favored against absence of change, revealing a relationship between mutation and decline in CD4 T-cell counts. Lastly, the exclusion of analysis of interim visits did not affect the results of overall CD4 T-cell decline in relatioship to the mapping of the evolution of HIV

References

  • Markham, R.B., Wang, W.C., Weisstein, A.E., Wang, Z., Munoz, A., Templeton, A., Margolick, J., Vlahov, D., Quinn, T., Farzadegan, H., & Yu, X.F. (1998). Patterns of HIV-1 evolution in individuals with differing rates of CD4 T cell decline. Proc Natl Acad Sci U S A. 95, 12568-12573. doi: 10.1073/pnas.95.21.12568 (PubMed ID: 9770526)
  • OpenWetWare. (2020). BIOL368/S20:Week 6. Retrieved February 20, 2020, from https://openwetware.org/wiki/BIOL368/S20:Week_6
  • Phylogeny.fr. (2020) Phylogeny.fr:Home. Retrieved February 20, 2020, from http://www.phylogeny.fr/.

Aknowledgement

  • I copied and modified directions and protocol from Week 6.
  • I worked with Karina Vescio and Sahil in class and outside of class to analyze data and create a presentation.
  • Copied and pasted nucleotide sequences to create phylogenetic trees from PubMed.
  • Made phylogenetic trees and sequence Clustal forms via Phylogeny.fr
  • Used data provieded in Nucleotide Sequence Data
  • Communicated for guidance in class and outside class Kam Dahlquist in how to proceed with our project.
  • Except for what is noted above, this individual journal entry was completed by me and not copied from another source. Lurbinah (talk) 23:39, 26 February 2020 (PST)