IGEM:UC Berkeley/2006

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Addressable Conjugation in Bacterial Networks

Networks of interacting cells provide the basis for neural learning. We have developed the process of addressable conjugation for communication within a network of E. coli bacteria. Here, bacteria send messages to one another via conjugation of plasmid DNAs, but the message is only meaningful to cells with a matching address sequence. In this way, the Watson Crick base-pairing of addressing sequences replaces the spatial connectivity present in neural systems. To construct this system, we have adapted natural conjugation systems as the communication device. Information contained in the transferred plasmids is only accessable by "unlocking" the message using RNA based 'keys'. The resulting addressable conjugation process is being adapted to construct a network of NAND logic gates in bacterial cultures. Ultimately, this will allow us to develop networks of bacteria capable of trained learning.
The presentation of our results for the 2006 Jamboree is available at the iGEM Website

The Berkeley iGEM team very gratefully acknowledges the generous support of Microsoft, which helped make our team members' participation possible.

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Bryan Hernandez
Matt Fleming
Kaitlin A. Davis
Jennifer Lu
Samantha Liang
Daniel Kluesing
Will Bosworth


John Dueber
Chris Anderson

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lab calendar

The Details

Construction Files
-80 Stocks


Procedure Overview (Plasmid DNA to Sequencing)
Competent cell production
Plasmid Transformation
Overlap extension of oligos
PCR prep
DNA Gel pouring
DNA purifications
PCR machine program (Expand kit)
Knockouts by Electroporation of pOX38 x pKD46

Subgroup Notebooks

Conjugation 6/7/06-7/21/06
Conjugation 7/24/06 - 09/07/06
Conjugation 09/07/06- present
Locks and Keys
Logic Gates
Bryan's Lock and Keys
Samantha's Notebook

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Addressable Cell-to-Cell Communication

Useful Links

Subgroup Strategies, Overview