IGEM:UC Berkeley/2006/DNAGelPrep
From OpenWetWare
Jump to navigationJump to search
Pouring Gels
- Fill 500ml beaker to ~350ml with cold water
- Mix in 6g agarose
- Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear
- Add 10ml 50x TAE Buffer
- Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing
- Add 50ul 10,000x SYBR Green (Bottle says ‘SYBR Safe DNA gel stain’, and liquid is very red)
- Pour into gel mold
-DLK_070306