IGEM:UC Berkeley/2006/DNAGelPrep

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Pouring Gels

  1. Fill 500ml beaker to ~350ml with cold water
  2. Mix in 6g agarose
  3. Give to chris to microwave, or if feeling brave, 30second intervals lightly swirling in between until liquid is clear
  4. Add 10ml 50x TAE Buffer
  5. Fill 500ml beaker to 600ml with cold h20, so a bit above the 500 mark but not so over flowing
  6. Add 50ul 10,000x SYBR Green (Bottle says ‘SYBR Safe DNA gel stain’, and liquid is very red)
  7. Pour into gel mold