IGEM:Stanford/2009/Project Homeostasis/sequences

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Project
Research Proposal
Systems Overview
Cloning Plan
Sequences & Primers
Anti-Inflammation
Device Overview
Parts Design
Challenges
Results
Anti-Immunosuppression
Device Overview
Parts Design
Challenges
Results
Protocols
Modeling
Overview
Notebook
Results
Future Work
Archived Work



Device 1

RBS – SoxR gene – Terminator – Spacer – SoxS Promoter click here


SoxR gene click here


SoxS Promoter click here


RBS - Blh gene click here


RBS - RALDH II gene - Terminator click here


hIL-6 hlyAs click here


Primer Design Guidelines


Resources

  • National Center for Biotechnology Information (NCBI)- Get Your Sequences

Home Page

  • Parts Registry of BioBricks- Help with Prefix and Suffix

Prefix and Suffix Help

  • IDT Integrated DNA Technologies- Check for %GC (40-60) and Melting Temp. (52-68C)

Analyze Your Sequence

  • NUPACK Nucleic Acid Package- check both primers for hairpins and trouble spots (No Red!!)

Analzye both Primers

  • ApE- A Plasmid Editor- Useful tool for checking sequences and finding reverse complements

Download here

  • Ginkgo BioWorks- creates primers w/ BioBrick additions

Design Primers Here


Archived Documents and Work