IGEM:Stanford/2009/Project Homeostasis/sequences/RaldhII

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RBS - RALDH II gene - Terminator

  • Sequence: length= 1732

gaattcgcggccgcttctagagaaagaggagaaatactagATGCCCGGCGAGGTGAAGGCCGACCCCGCCGCGCTC ATGGCCTCGCTGCAACTCCTGCCGTCACCCACGCCCAACCTCGAGATCAAGTACACCAAGATCTTTATT AACAATGAATGGCAGAACTCAGAGAGTGGGAGAGTGTTCCCTGTCTGTAATCCAGCCACAGGAGAGCAA GTGTGTGAAGTTCAAGAAGCAGACAAGGTGGATATAGACAAGGCAGTGCAGGCGGCCCGCCTGGCCTTC TCTCTTGGTTCTGTGTGGAGAAGGATGGACGCTTCTGAAAGAGGACGTCTGCTGGACAAGCTTGCAGACT TGGTGGAACGGGACAGGGCAACTCTTGCAACTATGGAATCACTAAATGGCGGTAAGCCATTCCTGCAAGC TTTTTACATCGATTTGCAGGGAGTCATCAAAACCCTGAGGTATTATGCAGGCTGGGCTGATAAAATTCACG GAATGACCATTCCTGTAGATGGAGACTATTTTACCTTCACGAGACATGAGCCCATCGGAGTGTGTGGACA GATTATCCCGTGGAATTTCCCCCTGCTGATGTTCACCTGGAAAATTGCTCCCGCACTGTGCTGTGGTAACA CTGTGGTCATCAAGCCGGCAGAGCAGACACCACTCAGTGCACTGTATATGGGAGCCCTCATCAAGGAGGC TGGCTTTCCACCCGGGGTCGTCAACATTCTGCCAGGGTATGGGCCAACAGCAGGGGCAGCAATAGCGTCT CACATTGGCATAGACAAGATTGCATTCACAGGCTCTACTGAGGTTGGAAAGCTTATTCAAGAAGCAGCTGG AAGAAGTAATTTGAAGAGAGTAACTCTGGAACTTGGAGGGAAAAGCCCTAATATTATTTTTGCAGATGCTG ACTTGGACTACGCTGTGGAGCAGGCTCACCAGGGTGTGTTCTTCAACCAAGGCCAGTGCTGCACTGCGGG GTCTCGAATCTTTGTGGAGGAGTCCATCTATGAGGAATTTGTGAAAAGAAGTGTGGAAAGAGCCAAGAGGC GCATTGTGGGGAGCCCCTTTGATCCCACTACTGAGCAGGGTCCTCAGATTGATAAGAAGCAATACAACAAA ATCCTGGAGCTCATCCAGAGCGGTGTGGCCGAGGGCGCCAAGCTCGAGTGTGGAGGCAAAGGACTGGGAA GGAAGGGCTTCTTCATTGAGCCTACCGTGTTCTCCAACGTTACAGATGACATGCGGATCGCCAAGGAGGAG ATCTTTGGTCCTGTCCAGGAAATTTTGAGGTTTAAGACTATGGATGAAGTTATAGAAAGAGCCAATAACTCA GACTTCGGGCTTGTAGCGGCTGTCTTCACTAATGACATCAACAAGGCTCTCATGGTGTCCTCTGCAATGCAA GCTGGGACAGTTTGGATCAATTGTTACAACGCCTTAAATGCTCAGAGCCCCTTCGGGGGGTTCAAGATGTCT GGAAATGGGAGAGAAATGGGTGAGTTTGGCTTACGGGAGTACTCAGAAGTAAAGACCGTGACGGTGAAGAT CCCCCAGAAGAACTCCTAAtactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttg tcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatactgcagcggccgctactagta


  • Forward Primer: gtttcttcgaattcgcggccgcttctagaaagaggagaaatactagatgcccgg
    • Length= 54 GC= 50% Melt= 69.9C


  • Reverse Primer: gtttcttcctgcagcggccgctactagtatataaacgcagaaaggcccacccg
    • Length= 53 GC= 54.7% Melt= 71C



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