IGEM:Stanford/2009/Project Homeostasis/sequences/Blh

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RBS - Blh gene

Sequence: length= 889

gaattcgcggccgcttctagagaaagaggagaaatactagATGGGTCTGATGCTGATTGATTGGTGTGCACTG GCTCTGGTTGTTTTCATTGGCCTGCCGCACGGCGCGCTGGATGCTGCCATTTCTTTTTCTATGAT CTCTTCTGCAAAACGCATTGCTCGTCTGGCTGGTATTCTGCTGATCTATCTGCTGCTGGCGACCG CGTTCTTCCTGATCTGGTATCAGCTGCCAGCGTTTAGCCTGCTGATCTTCCTGCTGATCTCCATT ATCCACTTTGGTATGGCAGACTTCAACGCGTCCCCAAGCAAACTGAAATGGCCGCATATCATCGC CCACGGCGGTGTTGTTACTGTTTGGCTGCCGCTGATCCAGAAAAACGAAGTAACTAAACTGTTTA GCATCCTGACTAACGGTCCGACTCCGATCCTGTGGGACATCCTGCTGATTTTCTTCCTGTGTTGG TCTATTGGCGTGTGTCTGCACACGTACGAAACCCTGCGCTCTAAACATTACAACATCGCCTTTGA ACTGATCGGTCTGATTTTCCTGGCGTGGTATGCGCCGCCTCTGGTTACGTTTGCCACTTACTTCTG CTTCATTCATTCCCGTCGCCACTTCTCCTTTGTGTGGAAGCAGCTGCAACACATGTCTTCCAAAAA GATGATGATTGGCAGCGCGATTATCCTGTCCTGTACCTCTTGGCTGATCGGCGGTGGTATCTATTT CTTCCTGAACTCCAAAATGATCGCCTCTGAGGCTGCGCTGCAAACTGTGTTCATCGGTCTGGCGGC ACTGACCGTGCCGCACATGATTCTGATCGACTTCATCTTCCGTCCGCACTCTTCCCGTATCAAAATC

AAAAACTGActgcagcggccgctactagta


  • Forward Primer: gtttcttcgaattcgcggccgcttctagaaagaggagaaatactagatgggtctgatg
    • Length= 58 GC= 46.6% Melt= 68.2C


  • Reverse Primer: gtttcttcctgcagcggccgctactagtatcagtttttgattttgatacgggaagagtgcgg
    • Length= 62 GC= 48.4% Melt= 69.9C


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