IGEM:Stanford/2009/Project Homeostasis/sequences/SoxRS

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RBS – SoxR gene – Terminator – Spacer – SoxS Promoter

  • Sequence: length= 799

gaattcgcggccgcttctagagaaagaggagaaatactagATGGAAAAGAAATTACCCCGCATTAAAG CGCTGCTAACCCCCGGCGAAGTGGCGAAACGCAGCGGTGTGGCGGTATCGGCGCTGCAT TTCTATGAAAGTAAAGGGTTGATTACCAGTATCCGTAACAGCGGCAATCAGCGGCGATAT AAACGTGATGTGTTGCGATATGTTGCAATTATCAAAATTGCTCAGCGTATTGGCATTCCG CTGGCGACCATTGGTGAAGCGTTTGGCGTGTTGCCCGAAGGGCATACGTTAAGTGCGAAA GAGTGGAAACAGCTTTCGTCCCAATGGCGAGAAGAGTTGGATCGGCGCATTCATACCTTA GTGGCGCTGCGTGACGAACTGGACGGATGTATTGGTTGTGGCTGCCTTTCGCGCAGTGAT TGCCCGTTGCGTAACCCGGGCGACCGCTTAGGAGAAGAAGGTACCGGCGCACGCTTGCT GGAAGATGAACAAAACTAAtactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatata ctagaggttctgttaagtaactgaacccaatgtcgttagtgacgcttacctcttaagaggtcactgacctaacaAATCGC TTTACCTCAAGTTAACTTGAGGAATTATACTCCCCAACAGATGAATTAACGAACTGctgcag

cggccgctactagta


  • Forward Primer: gtttcttcgaattcgcggccgcttctagaaagaggagaaatactagatgg
    • Length= 50 GC= 46% Melt= 66.7C


  • Reverse Primer: gtttcttcctgcagcggccgctactagtacagttcgttaattcatctgttggggag
    • Length= 56 GC= 50% Melt= 69.5C


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