IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation
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| Super Parts | Molecular Prey-Predator Oscillator | |
|---|---|---|
| Actual Part | 
| |
| Sub Parts | Test Sensing Predator Construct | Test Killing Predator Construct |
Ligation strategy
Ligating the Polycystronic Predator Construct:
| For | Stage 1 | Stage 2 | Stage 3 | Stage 4 |
|---|---|---|---|---|
(<bbpart>"PCR'd"</bbpart>) |
 |
|||
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>) |
 Error creating thumbnail: Unable to save thumbnail to destination |
|||
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>) |
 |
|||
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>) |
Error creating thumbnail: Unable to save thumbnail to destination |
|||
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>) |
 Error creating thumbnail: Unable to save thumbnail to destination |
|||
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>) |
 |
Ligating the Monocystronic Predator Construct:
- More information on ligations can be found on the Ligation Page.
Quality control
| Part | Logo | Built | Sequenced | Primers Used for Sequencing |
|---|---|---|---|---|
 |
No | N/A | N/A | |
 |
Yes | Yes | Primers used | |
|
Yes | Yes Note: This part was sequenced as a part of J37025 |
Primers used | |
|
No | N/A | N/A | |
 |
Yes | Yes | Primers used | |
 |
Yes | No | N/A | |
 |
Yes | No | N/A | |
 |
No | N/A | N/A | |
 |
No | N/A | N/A |
WetLab work entries
- the final assembly was not successful
- The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
- Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).
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