IGEM:IMPERIAL/2006/project/Oscillator/project browser/Predator Construct/Implementation

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Super Parts Molecular Prey-Predator Oscillator
Actual Part
Sub Parts Test Sensing Predator Construct Test Killing Predator Construct


Ligation strategy

Ligating the Polycystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37023</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>J37024</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37036</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>J37025</bbpart>)


Ligating the Monocystronic Predator Construct:

For Stage 1 Stage 2 Stage 3 Stage 4 Stage 5
<bbpart>J37023</bbpart>
(<bbpart>"PCR'd"</bbpart>)
<bbpart>J37024</bbpart>=
(<bbpart>J37023</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37025</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>J37024</bbpart>)
<bbpart>J37033</bbpart>=
(<bbpart>B0034</bbpart>+<bbpart>C0062</bbpart>)
<bbpart>J37019</bbpart>=
(<bbpart>R0062</bbpart>+<bbpart>J37033</bbpart>)
<bbpart>J37031</bbpart>=
(<bbpart>J37019</bbpart>+<bbpart>B0014</bbpart>)
<bbpart>J37018</bbpart>=
(<bbpart>J37031</bbpart>+<bbpart>R0062</bbpart>)
<bbpart>J37035</bbpart>=
(<bbpart>J37018</bbpart>+<bbpart>J37025</bbpart>)

Quality control

Part Logo Built Sequenced Primers Used for Sequencing
<bbpart>J37018</bbpart>
 No N/A N/A
<bbpart>J37019</bbpart>
 Yes Yes Primers used
<bbpart>J37023</bbpart>
 Yes Yes
Note: This part was sequenced as a part of J37025		 Primers used
<bbpart>J37024</bbpart>
 No N/A N/A
<bbpart>J37025</bbpart>
 Yes Yes Primers used
<bbpart>J37031</bbpart>
 Yes No N/A
<bbpart>J37033</bbpart>
 Yes No N/A
<bbpart>J37035</bbpart>
 No N/A N/A
<bbpart>J37036</bbpart>
 No N/A N/A

WetLab work entries

  • the final assembly was not successful
  • The AiiA gene available from the registry seemed non-functional. We sourced new AiiA to which we then PCR'd LVA-tag and immunotag. See design section for this part for more information]
  • Reasons for parts not being built include time constraint (and perhaps non-functional registry parts).

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