The purpouse for building and testing this part is so we can characterize the production of AHL-lactonase
The Aiia gene has been replaced with GFP to allow direct measurement of protein production
From this data we can create a transfer function where AHL is the input and GFP the output
Making the assumpion that AHL-lactonase is transcribed at the same rate as GFP we can use the transfer curve to extract parameters to allow characterization of the sensing part of the predator
The part will be used to form the sensing part of the final predator construct
Parts Interface
INPUTS
OUTPUTS
AHL
AHL-lactonase & fluctuating [AHL]
Predator construct at the device level.
INPUTS
OUTPUTS
AHL
AHL-lactonase
Predator sensing test construct.
INPUTS
OUTPUTS
AHL
GFP
Predator sensing test construct.
Expected behavior and performances
System level specifications
Properties
Specifications
Input Range(s)
[0.01nm,10000nm]
Output Range(s)
?
Transfer function(s)
Robustness
Device should work for at least 10 cycles of the oscillator to match system level oscillator specifications.
Variability
Variability at the device population level (day to day, device to device) should be as low as possible.
