IGEM:IMPERIAL/2006/project/parts/Primers

Primers

The primers are used to assess whether ligations have been successful. This is achieved by combining a primer complimentary to the desired insert in one orientation, coupled with a primer for the appropriate plasmid in the other orientation.

Part Primers

Location of Primer sequences within parts to be found here

Forward

• I13504
  • Sequence: CAA CTA GCA GAC CAT TAT C
  • Secondary Strucure: None

• C0062 1
  • Sequence: CAT GCG TGT ATG AAC AC
  • Secondary Strucure: Moderate

• C0062 2
  • Sequence: TAA CAT CAG GTC TTA TC
  • Secondary Strucure: None

• I13033
  • Sequence: CTG TAG GAT CGT ACA GG
  • Secondary Strucure: Moderate

• J37023
  • Sequence: TGC TGA ATA TGA GGC GG
  • Secondary Strucure: None

Reverse

• Riboswitch Primer
  • Sequence: TCA TCT TTT GTT TAA TC
  • Secondary Strucure: None

• R0062+B0034 Primer
  • Sequence: GCG TAA ACC TGT ACG ATC C
  • Secondary Strucure: Moderate

Plasmid Primers

Reverse

• pSB1(A2/A3/AK3)
  • Sequence: TGA TTC TGT GGA TAA CCG
  • Seconday Structure: None

• pSB1(AK3/A3)
  • Sequence: CGA GGA AGC CTG CAT AAC G
  • Secondary Structure: Weak

Forward

• pSB1(AK3/A3/A2)
  • Sequence: TAT TGT CTC ATG AGC GG
  • Seconday Structure: None

• pSB1A2 Primer (For use with Riboswitch + Construct)
  • Sequence: CAG AAG TAA GTT GGC CGC
  • Secondary Structure: None

Oligonucleotide Constructs

• Riboswitch + Strand
  • Sequence: AAT TCG CGG CCG CAT CTA GAG ATA CTT AAT TTA AAG ATT AAA CAA AAG ATG ATA CCA GCC GAA AGG CCC TTG GCA GCT CTC GTG GAG TGT A

• Riboswitch - Strand
  • Sequence: CTA GTA CAC TCC ACG AGA GCT GCC AAG GGC CTT TGG GCT GGT ATC ATC TTT TGT TTA ATC TTT AAA TTA AGT ATC TCT AGA TGC GGC CGC G

• R0062+B0034 Construct + Strand
  • Sequence: AAT TCG CGG CCG CTT CTA GAG ACC TGT AGG ATC GTA CAG GTT TAC GCA AGA AAA TGG TTT GTT ATA GTC GAA TAA ATA CTA GAA AGA GGA GAA ATA

• R0062+B0034 Construct - Strand
  • Sequence: CTA GTA TTT CTC CTC TTT CTA GTA TTT ATT CGA CTA TAA CAA ACC ATT TTC TTG CGT AAA CCT GTA CGA TCC TAC AGG TCT CTA GAA GCG GCC GCG

• Immuno Tag Part 1
  • Sequence: GAA TTC GCG GCC GCT TCT AGA GGA TTA TAA AGA TGA TGA TGA TAA AGG TAT GAC AGT AAA GAA GCT TTA T

• Immuno Tag Part 2
  • Sequence: CTG CAG CGG CCG CTA CTA GTA TTA TTA ACG TAC TAA