From OpenWetWare
Jump to navigationJump to search


About Us       Protocols &c.       Lab Members       Publications       Contact       Links

Welcome to the Dionne lab!

That is to say, Marc Dionne's lab, at Imperial College London; not to be confused with any other Dionne lab.

We are interested in the ways in which host genotype affects the biology of bacterial infection. Drosophila melanogaster is our animal model of choice.

Work in the lab has been funded by the Biotechnology and Biological Sciences Research Council, the Wellcome Trust, and the Medical Research Council.

A BBSRC-funded postdoctoral position is available in the lab! If you're interested, get in touch, or apply here!

Metabolic-immune interaction and host genetics in infection

It has been known for centuries that chronic infections cause systemic metabolic disruption, but it is fundamentally unclear why and how these events are linked. Does metabolic disruption somehow facilitate the host response to infection? If so, how? We address these fundamental biological questions by analyzing pathogenic infections and their consequences in the fruit-fly Drosophila melanogaster. We use classical Drosophila genetics, computational analysis and modeling of gene expression, biochemistry, and intravital microscopy to probe the metabolic-immune interface.

One pathogen of particular interest to us is Mycobacterium marinum. We have previously shown that flies infected with M. marinum exhibit progressive loss of metabolic stores accompanied by mild hyperglycemia. We have shown that these effects are caused, in part, by systemic disruption of signaling via the anabolic effector kinases Akt and p70 S6 kinase. The transcription factor MEF2 responds to nutrient signals to regulate expression of both immune effectors and anabolic enzymes. Remarkably, though MEF2 promotes the expression of both groups of genes, its choice of targets is regulated by a conserved phosphorylation that alters its affinity for the TATA binding protein. It appears that the disruption of anabolic kinase activity may be required to permit MEF2 to drive the antibacterial response. This work has recently been published in Cell.

Ongoing work continues to explore other metabolic inputs into MEF2, other targets of MEF2 in its two discrete physiological states, and the pathways by which infection disrupts anabolic kinase activity.

Cytokines and cytokine signalling

In the course of screening for mutants with defective responses to M. marinum, we find a lot of molecules and pathways that end up being involved in cytokine signalling and its consequences. Cytokines regulate the realized immune response of the fly, much as they do in mammals; they also can be significant direct drivers of pathology due to effects on immune and nonimmune target tissues. However, very little is known about the biology of cytokines in Drosophila melanogaster, especially in the context of bacterial infections.

Some time back, we showed that two different TGF-betas regulate fly immunity, each inhibiting a specific arm of the immune response, and each being produced by only a subset of phagocytes. Check it out!

We've also done some exciting work in collaboration with Frederic Geissmann's lab on the role of JAK-STAT signalling in flies on a high-fat diet; you can read about it here. We continue to work on the roles of this pathway in Mycobacterium marinum infection and in muscle physiology—we hope to be able to say more about these soon.


Recent updates to the lab wiki

List of abbreviations:
This edit created a new page (also see list of new pages)
This is a minor edit
This edit was performed by a bot
The page size changed by this number of bytes

25 March 2023

     12:29  Capillary Number - Christopher Sparages‎‎ 5 changes history +550 [Joebijujosep‎ (5×)]
12:29 (cur | prev) +66 Joebijujosep talk contribs (→‎Introduction)
12:04 (cur | prev) +222 Joebijujosep talk contribs (→‎Applications)
11:57 (cur | prev) +204 Joebijujosep talk contribs (→‎Applications)
11:53 (cur | prev) +51 Joebijujosep talk contribs (→‎Capillary Number Theory)
11:12 (cur | prev) +7 Joebijujosep talk contribs (→‎Introduction)
     12:12  Paperfuge - Anson Staples & Ian Taylor diffhist −1 Gjschneider talk contribs
     11:22  File:CO2 Storage CO2GeoNet.png‎‎ 2 changes history +144 [Joebijujosep‎ (2×)]
11:22 (cur | prev) +58 Joebijujosep talk contribs (→‎Summary)
11:21 (cur | prev) +86 Joebijujosep talk contribs (→‎Summary)
     11:20  (Upload log) [Joebijujosep‎ (2×)]
11:20 Joebijujosep talk contribs uploaded File:CO2 Storage CO2GeoNet.png(CO2 Storage Processes)
10:48 Joebijujosep talk contribs uploaded File:Capillary Number Table.jpg(Reproduced version of Capillary Number from literature. https://perminc.com/resources/fundamentals-of-fluid-flow-in-porous-media/chapter-2-the-porous-medium/multi-phase-saturated-rock-properties/dominance-capillary-forces-viscous-forces/capillary-number/)
     03:59  Magni:Lab Members diffhist +375 Lorenzo Pasotti talk contribs

24 March 2023

N    22:42  Microfluidics and Microgravity diffhist +25 Egboni talk contribs (Created page with "{{Template:CHEM-ENG509E}}")
     22:30  Wet Etching - Eric Ying‎‎ 2 changes history 0 [Egboni‎ (2×)]
22:30 (cur | prev) 0 Egboni talk contribs (→‎Isotropic Etching) Tag: Manual revert
22:30 (cur | prev) 0 Egboni talk contribs (→‎Isotropic Etching)
     14:22  Paperfuge - Anson Staples & Ian Taylor‎‎ 4 changes history +94 [Gjschneider‎ (4×)]
14:22 (cur | prev) −1 Gjschneider talk contribs
14:21 (cur | prev) −4 Gjschneider talk contribs
14:15 (cur | prev) 0 Gjschneider talk contribs
13:51 (cur | prev) +99 Gjschneider talk contribs
 m   13:55  Dry Etching - Kevin Lang diffhist −1 Gmcgregor19 talk contribs (Correctly ordering footnotes/references, minor grammatical fixes)
     09:05  Sterilizer diffhist +332 Marc Perez talk contribs (→‎AMSCO Sterilizers)
 m   03:20  User:Richard J. Abbott‎‎ 2 changes history +241 [Richard J. Abbott‎ (2×)]
03:20 (cur | prev) −37 Richard J. Abbott talk contribs (→‎Publications)
03:16 (cur | prev) +278 Richard J. Abbott talk contribs (→‎Publications)

23 March 2023

     19:36  Spin Coating - Yizhuo Chen‎‎ 5 changes history −26 [Wwwong‎ (5×)]
19:36 (cur | prev) +33 Wwwong talk contribs (→‎References)
19:28 (cur | prev) −28 Wwwong talk contribs (→‎PDMS)
19:26 (cur | prev) −14 Wwwong talk contribs (→‎PDMS)
19:23 (cur | prev) −18 Wwwong talk contribs (→‎Introduction to Spin Coating)
19:22 (cur | prev) +1 Wwwong talk contribs (→‎Method)

22 March 2023

     21:51  User:Shantanu Ghosh‎‎ 5 changes history −38 [Shantanu Ghosh‎ (5×)]
21:51 (cur | prev) +11 Shantanu Ghosh talk contribs (→‎Current projects)
21:51 (cur | prev) −25 Shantanu Ghosh talk contribs (→‎My current obsession)
21:50 (cur | prev) +11 Shantanu Ghosh talk contribs (→‎Current projects)
21:49 (cur | prev) +9 Shantanu Ghosh talk contribs (→‎My current obsession)
21:34 (cur | prev) −44 Shantanu Ghosh talk contribs
     15:43  Thermophoresis, Microscale‎‎ 2 changes history +181 [Marc Perez‎ (2×)]
15:43 (cur | prev) +174 Marc Perez talk contribs (→‎Monolith NT.115 (B413))
15:38 (cur | prev) +7 Marc Perez talk contribs (→‎Monolith NT.115)
     15:34  Warm & Cold Room Practices diffhist +81 Marc Perez talk contribs (→‎WARM & COLDROOM PRACTICES)
     15:32  Water Purification, Aqua Solutions‎‎ 2 changes history +71 [Marc Perez‎ (2×)]
15:32 (cur | prev) −28 Marc Perez talk contribs (→‎AQUA SOLUTIONS WATER PURIFICATION (B414 & B432))
15:30 (cur | prev) +99 Marc Perez talk contribs (→‎AQUA SOLUTIONS WATER PURIFICATION (B414 & B432))
     15:28  Wet Chemistry Room diffhist +103 Marc Perez talk contribs (→‎CHEMISTRY SAFETY)
     15:26  X-Ray Film Processor‎‎ 2 changes history +94 [Marc Perez‎ (2×)]
15:26 (cur | prev) +48 Marc Perez talk contribs (→‎X-Ray Film Processor (B452))
15:24 (cur | prev) +46 Marc Perez talk contribs (→‎X-Ray Film Processor (B452))
     09:22  McClean:Protocols‎‎ 4 changes history −35 [Rjszakaly‎ (4×)]
09:22 (cur | prev) −106 Rjszakaly talk contribs (→‎Drugs)
09:21 (cur | prev) +46 Rjszakaly talk contribs (→‎Media)
09:20 (cur | prev) +60 Rjszakaly talk contribs (→‎Media)
09:19 (cur | prev) −35 Rjszakaly talk contribs (→‎Drugs)

21 March 2023

20 March 2023

     14:14  CHIP:Talks diffhist 0 Gabor Balazsi talk contribs
     06:31  Hu:Publications diffhist +11 Hugangqing talk contribs

19 March 2023


<nonwikionly> This page was created using Open Wetware.</nonwikionly>