Dionne

From OpenWetWare
Jump to navigationJump to search

Imperial-banner.png

About Us       Protocols &c.       Lab Members       Publications       Contact       Links


Welcome to the Dionne lab!

That is to say, Marc Dionne's lab, at Imperial College London; not to be confused with any other Dionne lab.

We are interested in the ways in which host genotype affects the biology of bacterial infection. Drosophila melanogaster is our animal model of choice.

Work in the lab has been funded by the Biotechnology and Biological Sciences Research Council, the Wellcome Trust, and the Medical Research Council.

A BBSRC-funded postdoctoral position is available in the lab! If you're interested, get in touch, or apply here!

Metabolic-immune interaction and host genetics in infection

It has been known for centuries that chronic infections cause systemic metabolic disruption, but it is fundamentally unclear why and how these events are linked. Does metabolic disruption somehow facilitate the host response to infection? If so, how? We address these fundamental biological questions by analyzing pathogenic infections and their consequences in the fruit-fly Drosophila melanogaster. We use classical Drosophila genetics, computational analysis and modeling of gene expression, biochemistry, and intravital microscopy to probe the metabolic-immune interface.

One pathogen of particular interest to us is Mycobacterium marinum. We have previously shown that flies infected with M. marinum exhibit progressive loss of metabolic stores accompanied by mild hyperglycemia. We have shown that these effects are caused, in part, by systemic disruption of signaling via the anabolic effector kinases Akt and p70 S6 kinase. The transcription factor MEF2 responds to nutrient signals to regulate expression of both immune effectors and anabolic enzymes. Remarkably, though MEF2 promotes the expression of both groups of genes, its choice of targets is regulated by a conserved phosphorylation that alters its affinity for the TATA binding protein. It appears that the disruption of anabolic kinase activity may be required to permit MEF2 to drive the antibacterial response. This work has recently been published in Cell.

Ongoing work continues to explore other metabolic inputs into MEF2, other targets of MEF2 in its two discrete physiological states, and the pathways by which infection disrupts anabolic kinase activity.

Cytokines and cytokine signalling

In the course of screening for mutants with defective responses to M. marinum, we find a lot of molecules and pathways that end up being involved in cytokine signalling and its consequences. Cytokines regulate the realized immune response of the fly, much as they do in mammals; they also can be significant direct drivers of pathology due to effects on immune and nonimmune target tissues. However, very little is known about the biology of cytokines in Drosophila melanogaster, especially in the context of bacterial infections.

Some time back, we showed that two different TGF-betas regulate fly immunity, each inhibiting a specific arm of the immune response, and each being produced by only a subset of phagocytes. Check it out!

We've also done some exciting work in collaboration with Frederic Geissmann's lab on the role of JAK-STAT signalling in flies on a high-fat diet; you can read about it here. We continue to work on the roles of this pathway in Mycobacterium marinum infection and in muscle physiology—we hope to be able to say more about these soon.

<wikionly>

Recent updates to the lab wiki

List of abbreviations:
N
This edit created a new page (also see list of new pages)
m
This is a minor edit
b
This edit was performed by a bot
(±123)
The page size changed by this number of bytes

19 January 2021

18 January 2021

     23:25  Lee:JC diffhist +45 Wooin Lee talk contribs →‎Past papers
     20:10  Taylor Makela Journal Week 11 diffhist +1,965 Taylor Makela talk contribs →‎Discussion: added discussion section
     12:38  Synthetic mycology‎‎ 2 changes history -1 [Rolando Cruz Perez‎ (2×)]
     
12:38 (cur | prev) -52 Rolando Cruz Perez talk contribs →‎Mycology Tags: Mobile edit Mobile web edit
     
12:36 (cur | prev) +51 Rolando Cruz Perez talk contribs →‎Mycology Tags: Mobile edit Mobile web edit
     07:10  WAKNA:Basics‎‎ 2 changes history +418 [Berthold Drexler‎ (2×)]
     
07:10 (cur | prev) 0 Berthold Drexler talk contribs →‎Hier finden Sie Literatur für Einsteiger:innen in das Gebiet der Neuroanästhesie
     
07:09 (cur | prev) +418 Berthold Drexler talk contribs →‎intracranielle Blutung
     06:39  Silver: 2021 Meeting Schedule‎‎ 2 changes history 0 [PamSilver‎ (2×)]
     
06:39 (cur | prev) 0 PamSilver talk contribs →‎March
     
06:39 (cur | prev) 0 PamSilver talk contribs →‎January

17 January 2021

     14:22  Cornell SciFi Lab:Lab Members‎‎ 18 changes history +13,159 [TianlinZhao‎ (18×)]
     
14:22 (cur | prev) -94 TianlinZhao talk contribs
     
14:20 (cur | prev) +118 TianlinZhao talk contribs
     
14:17 (cur | prev) +6 TianlinZhao talk contribs
     
14:16 (cur | prev) +1 TianlinZhao talk contribs
     
14:15 (cur | prev) +607 TianlinZhao talk contribs
     
14:13 (cur | prev) -8 TianlinZhao talk contribs
     
14:12 (cur | prev) +1,097 TianlinZhao talk contribs
     
14:02 (cur | prev) +338 TianlinZhao talk contribs
     
14:01 (cur | prev) +708 TianlinZhao talk contribs
     
13:53 (cur | prev) +119 TianlinZhao talk contribs
     
13:36 (cur | prev) +112 TianlinZhao talk contribs
     
13:28 (cur | prev) +972 TianlinZhao talk contribs
     
13:21 (cur | prev) +1,767 TianlinZhao talk contribs
     
13:15 (cur | prev) +1,750 TianlinZhao talk contribs
     
13:08 (cur | prev) +16 TianlinZhao talk contribs
     
13:05 (cur | prev) -5 TianlinZhao talk contribs
     
12:55 (cur | prev) +3,909 TianlinZhao talk contribs
     
12:47 (cur | prev) +1,746 TianlinZhao talk contribs
     14:16  (Upload log) [TianlinZhao‎ (20×)]
     
14:16 TianlinZhao talk contribs uploaded File:Portrait Bo Liang.jpg
     
14:13 TianlinZhao talk contribs uploaded File:Portrait Zihan Yan.png
     
14:10 TianlinZhao talk contribs uploaded File:Portrait Chris Oh.png
     
13:21 TianlinZhao talk contribs uploaded File:Portrait Songlin Xu.JPG
     
13:20 TianlinZhao talk contribs uploaded File:Portrait Peng He.JPG
     
13:20 TianlinZhao talk contribs uploaded File:Portrait Fang Hu.JPG
     
13:15 TianlinZhao talk contribs uploaded File:Portrait Yaxuan Li.JPG
     
13:14 TianlinZhao talk contribs uploaded File:Portrait Wei Sun.JPG
     
13:14 TianlinZhao talk contribs uploaded File:Portrait Tuochao Chen.JPG
     
13:08 TianlinZhao talk contribs uploaded a new version of File:Portrait Tianlin Zhao.jpg
     
13:05 TianlinZhao talk contribs uploaded File:Portrait Kinan Alsheikh.JPG
     
13:05 TianlinZhao talk contribs uploaded File:Portrait Lucy Wang.JPG
     
13:04 TianlinZhao talk contribs uploaded File:Portrait Andrew Xu.JPG
     
13:04 TianlinZhao talk contribs uploaded File:Portrait Clara Song.JPG
     
13:04 TianlinZhao talk contribs uploaded File:Portrait Matthew Dressa.JPG
     
13:03 TianlinZhao talk contribs uploaded File:Portrait David Lin.JPG
     
13:03 TianlinZhao talk contribs uploaded File:Portrait Samhita Pendyal.JPG
     
13:02 TianlinZhao talk contribs uploaded File:Portrait Jessica Tweneboah.JPG
     
13:02 TianlinZhao talk contribs uploaded File:Portrait Tianlin Zhao.jpg
     
12:47 TianlinZhao talk contribs uploaded File:Portrait Benjamin Steeper.JPG

</wikionly>

<nonwikionly> This page was created using Open Wetware.</nonwikionly>