Dionne
Contents
Welcome to the Dionne lab!
That is to say, Marc Dionne's lab, at Imperial College London; not to be confused with any other Dionne lab.
We are interested in the ways in which host genotype affects the biology of bacterial infection. Drosophila melanogaster is our animal model of choice.
Work in the lab has been funded by the Biotechnology and Biological Sciences Research Council, the Wellcome Trust, and the Medical Research Council.
A BBSRC-funded postdoctoral position is available in the lab! If you're interested, get in touch, or apply here!
Metabolic-immune interaction and host genetics in infection
It has been known for centuries that chronic infections cause systemic metabolic disruption, but it is fundamentally unclear why and how these events are linked. Does metabolic disruption somehow facilitate the host response to infection? If so, how? We address these fundamental biological questions by analyzing pathogenic infections and their consequences in the fruit-fly Drosophila melanogaster. We use classical Drosophila genetics, computational analysis and modeling of gene expression, biochemistry, and intravital microscopy to probe the metabolic-immune interface.
One pathogen of particular interest to us is Mycobacterium marinum. We have previously shown that flies infected with M. marinum exhibit progressive loss of metabolic stores accompanied by mild hyperglycemia. We have shown that these effects are caused, in part, by systemic disruption of signaling via the anabolic effector kinases Akt and p70 S6 kinase. The transcription factor MEF2 responds to nutrient signals to regulate expression of both immune effectors and anabolic enzymes. Remarkably, though MEF2 promotes the expression of both groups of genes, its choice of targets is regulated by a conserved phosphorylation that alters its affinity for the TATA binding protein. It appears that the disruption of anabolic kinase activity may be required to permit MEF2 to drive the antibacterial response. This work has recently been published in Cell.
Ongoing work continues to explore other metabolic inputs into MEF2, other targets of MEF2 in its two discrete physiological states, and the pathways by which infection disrupts anabolic kinase activity.
Cytokines and cytokine signalling
In the course of screening for mutants with defective responses to M. marinum, we find a lot of molecules and pathways that end up being involved in cytokine signalling and its consequences. Cytokines regulate the realized immune response of the fly, much as they do in mammals; they also can be significant direct drivers of pathology due to effects on immune and nonimmune target tissues. However, very little is known about the biology of cytokines in Drosophila melanogaster, especially in the context of bacterial infections.
Some time back, we showed that two different TGF-betas regulate fly immunity, each inhibiting a specific arm of the immune response, and each being produced by only a subset of phagocytes. Check it out!
We've also done some exciting work in collaboration with Frederic Geissmann's lab on the role of JAK-STAT signalling in flies on a high-fat diet; you can read about it here. We continue to work on the roles of this pathway in Mycobacterium marinum infection and in muscle physiology—we hope to be able to say more about these soon.
<wikionly>
Recent updates to the lab wiki
- N
- This edit created a new page (also see list of new pages)
- m
- This is a minor edit
- b
- This edit was performed by a bot
- (±123)
- The page size changed by this number of bytes
24 March 2019
|
|
|
19:32 |
(User creation log) . . [Yar (2×)] |
||
|
|
19:32 . . User account DimKunFah (talk | contribs) was created by Yar (talk | contribs) and password was sent by email |
||||
|
|
10:55 . . User account Michaelhag (talk | contribs) was created by Yar (talk | contribs) and password was sent by email |
||||
|
|
|
N 17:41 |
Avalekander Week 9 (10 changes | history) . . (+1,877) . . [Avalekander (10×)] |
||
|
|
(cur | prev) . . (+7) . . Avalekander (talk | contribs) (→Acknowledgements: edited username link) |
||||
|
|
(cur | prev) . . (+1) . . Avalekander (talk | contribs) (→Acknowledgements) |
||||
|
|
(cur | prev) . . (-2) . . Avalekander (talk | contribs) (→Acknowledgements: fixed wiki format issue) |
||||
|
|
(cur | prev) . . (+284) . . Avalekander (talk | contribs) (→Acknowledgements: acknowledged homework partner and added statement "Except for what is noted above, this individual journal entry was completed by me and not copied from another source.") |
||||
|
|
(cur | prev) . . (-19) . . Avalekander (talk | contribs) (→Outline: fixed number format) |
||||
|
|
(cur | prev) . . (+1,373) . . Avalekander (talk | contribs) (→Outline: added questions to be answered) |
||||
|
|
(cur | prev) . . (+39) . . Avalekander (talk | contribs) (→Answers: added sub-headers) |
||||
|
|
(cur | prev) . . (+64) . . Avalekander (talk | contribs) (→Biology 388 Assignments: added sections) |
||||
|
|
(cur | prev) . . (+2) . . Avalekander (talk | contribs) (→Biology 388 Assignments: fixed format of category) |
||||
| N |
|
(cur | prev) . . (+128) . . Avalekander (talk | contribs) (added template and category) |
|||
| 17:38 | User:Avalekander (diff | hist) . . (+1) . . Avalekander (talk | contribs) (→Interests: altered rights to justice) | ||||
|
|
|
N 17:03 |
Leanne Kuwahara-Week 9 (2 changes | history) . . (+793) . . [Leannekuwahara (2×)] |
||
|
|
(cur | prev) . . (+148) . . Leannekuwahara (talk | contribs) (→Purpose: added purp) |
||||
| N |
|
(cur | prev) . . (+645) . . Leannekuwahara (talk | contribs) (Added sections for hw 9) |
|||
|
|
|
14:25 |
Schumer lab: Submitting slurm jobs (3 changes | history) . . (+651) . . [Schumer (3×)] |
||
|
|
|||||
|
|
(cur | prev) . . (+258) . . Schumer (talk | contribs) (→Lab specific nodes) |
||||
|
|
(cur | prev) . . (+1) . . Schumer (talk | contribs) (→Useful slurm commands) |
||||
|
|
|
14:17 |
Yeo lab:Reprints (3 changes | history) . . (+22) . . [Yoonyeo (3×)] |
||
|
|
(cur | prev) . . (+5) . . Yoonyeo (talk | contribs) (→2018) |
||||
|
|
(cur | prev) . . (0) . . Yoonyeo (talk | contribs) (→2019) |
||||
|
|
(cur | prev) . . (+17) . . Yoonyeo (talk | contribs) (→2019) |
||||
| N 08:21 | Schumer lab: slurm resources (diff | hist) . . (+126) . . Schumer (talk | contribs) (Created page with "Here are some great slurm resources: 1. https://ulhpc-tutorials.readthedocs.io/en/latest/scheduling/advanced/ ULHPC guide") | ||||
23 March 2019
|
|
|
22:53 |
User:Shantanu Ghosh (14 changes | history) . . (+103) . . [Shantanu Ghosh (14×)] |
||
|
|
(cur | prev) . . (+46) . . Shantanu Ghosh (talk | contribs) (→Current projects) |
||||
| m |
|
(cur | prev) . . (+39) . . Shantanu Ghosh (talk | contribs) |
|||
|
|
(cur | prev) . . (-13) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (0) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (+83) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (-1) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (+6) . . Shantanu Ghosh (talk | contribs) |
||||
| m |
|
(cur | prev) . . (0) . . Shantanu Ghosh (talk | contribs) |
|||
|
|
(cur | prev) . . (-1) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (0) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (0) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (-113) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (+66) . . Shantanu Ghosh (talk | contribs) |
||||
|
|
(cur | prev) . . (-9) . . Shantanu Ghosh (talk | contribs) |
||||
| 22:32 | BIOL388/S19:Class Journal Week 7 (diff | hist) . . (+880) . . Leannekuwahara (talk | contribs) (added leanne's response) | ||||
| 22:25 | (Upload log) . . Shantanu Ghosh (talk | contribs) uploaded File:450px-Ghoshwikipic-2.jpg | ||||
| 20:09 | (User creation log) . . User account Carlyswheen (talk | contribs) was created by Yar (talk | contribs) and password was sent by email | ||||
| 18:15 | DOs and DONTs of Good Lab Citizenship (diff | hist) . . (-11) . . Yar (talk | contribs) (don't BE a sexual predator and don't JOKE about it... thanks @ProfPhoebe https://twitter.com/ProfPhoebe/status/1109534883284606976) | ||||
|
|
|
11:35 |
BIOL388/S19 (3 changes | history) . . (-171) . . [Kam D. Dahlquist (3×)] |
||
|
|
(cur | prev) . . (+39) . . Kam D. Dahlquist (talk | contribs) (→Schedule: add research presentations to march 26) |
||||
|
|
(cur | prev) . . (0) . . Kam D. Dahlquist (talk | contribs) (→Schedule: moved credit/no credit deadline) |
||||
|
|
(cur | prev) . . (-210) . . Kam D. Dahlquist (talk | contribs) (→Upcoming Seminars: delete old seminar) |
||||
|
|
|
11:32 |
BIOL388/S19:Week 9 (7 changes | history) . . (+9,444) . . [Kam D. Dahlquist (7×)] |
||
|
|
(cur | prev) . . (+133) . . Kam D. Dahlquist (talk | contribs) (→Reflection: add reflection question) |
||||
|
|
(cur | prev) . . (-23) . . Kam D. Dahlquist (talk | contribs) |
||||
|
|
(cur | prev) . . (+63) . . Kam D. Dahlquist (talk | contribs) (→Preparation for Journal Club 2: assigned people) |
||||
|
|
(cur | prev) . . (+95) . . Kam D. Dahlquist (talk | contribs) (→Homework Partners: assigned homework partners) |
||||
|
|
(cur | prev) . . (-20) . . Kam D. Dahlquist (talk | contribs) (→Preparation for Journal Club 2: type in figures) |
||||
|
|
(cur | prev) . . (+326) . . Kam D. Dahlquist (talk | contribs) (→Preparation for Journal Club 2: add link to paper) |
||||
|
|
(cur | prev) . . (+8,870) . . Kam D. Dahlquist (talk | contribs) (copied over from week 3, changed to week 9) |
||||
</wikionly>
<nonwikionly> This page was created using Open Wetware.</nonwikionly>
