Dionne

From OpenWetWare
Jump to navigationJump to search

Imperial-banner.png

About Us       Protocols &c.       Lab Members       Publications       Contact       Links


Welcome to the Dionne lab!

That is to say, Marc Dionne's lab, at Imperial College London; not to be confused with any other Dionne lab.

We are interested in the ways in which host genotype affects the biology of bacterial infection. Drosophila melanogaster is our animal model of choice.

Work in the lab has been funded by the Biotechnology and Biological Sciences Research Council, the Wellcome Trust, and the Medical Research Council.

A BBSRC-funded postdoctoral position is available in the lab! If you're interested, get in touch, or apply here!

Metabolic-immune interaction and host genetics in infection

It has been known for centuries that chronic infections cause systemic metabolic disruption, but it is fundamentally unclear why and how these events are linked. Does metabolic disruption somehow facilitate the host response to infection? If so, how? We address these fundamental biological questions by analyzing pathogenic infections and their consequences in the fruit-fly Drosophila melanogaster. We use classical Drosophila genetics, computational analysis and modeling of gene expression, biochemistry, and intravital microscopy to probe the metabolic-immune interface.

One pathogen of particular interest to us is Mycobacterium marinum. We have previously shown that flies infected with M. marinum exhibit progressive loss of metabolic stores accompanied by mild hyperglycemia. We have shown that these effects are caused, in part, by systemic disruption of signaling via the anabolic effector kinases Akt and p70 S6 kinase. The transcription factor MEF2 responds to nutrient signals to regulate expression of both immune effectors and anabolic enzymes. Remarkably, though MEF2 promotes the expression of both groups of genes, its choice of targets is regulated by a conserved phosphorylation that alters its affinity for the TATA binding protein. It appears that the disruption of anabolic kinase activity may be required to permit MEF2 to drive the antibacterial response. This work has recently been published in Cell.

Ongoing work continues to explore other metabolic inputs into MEF2, other targets of MEF2 in its two discrete physiological states, and the pathways by which infection disrupts anabolic kinase activity.

Cytokines and cytokine signalling

In the course of screening for mutants with defective responses to M. marinum, we find a lot of molecules and pathways that end up being involved in cytokine signalling and its consequences. Cytokines regulate the realized immune response of the fly, much as they do in mammals; they also can be significant direct drivers of pathology due to effects on immune and nonimmune target tissues. However, very little is known about the biology of cytokines in Drosophila melanogaster, especially in the context of bacterial infections.

Some time back, we showed that two different TGF-betas regulate fly immunity, each inhibiting a specific arm of the immune response, and each being produced by only a subset of phagocytes. Check it out!

We've also done some exciting work in collaboration with Frederic Geissmann's lab on the role of JAK-STAT signalling in flies on a high-fat diet; you can read about it here. We continue to work on the roles of this pathway in Mycobacterium marinum infection and in muscle physiology—we hope to be able to say more about these soon.

<wikionly>

Recent updates to the lab wiki

List of abbreviations:
N
This edit created a new page (also see list of new pages)
m
This is a minor edit
b
This edit was performed by a bot
(±123)
The page size changed by this number of bytes

15 June 2021

     12:51  BioMicroCenter:People‎‎ 4 changes history +735 [Russov‎; Ospencer‎ (3×)]
     
12:51 (cur | prev) +178 Russov talk contribs →‎WET LAB
     
12:46 (cur | prev) 0 Ospencer talk contribs →‎WET LAB
     
12:46 (cur | prev) 0 Ospencer talk contribs →‎BioMicro Center Staff
     
12:44 (cur | prev) +557 Ospencer talk contribs →‎WET LAB
 m   11:51  Griffin:siRNA transfection‎‎ 4 changes history +655 [Korey Griffin‎ (4×)]
 m   
11:51 (cur | prev) -3 Korey Griffin talk contribs →‎siRNA transfection Procedure 24 well
 m   
11:51 (cur | prev) +220 Korey Griffin talk contribs →‎siRNA transfection Procedure 24 well
 m   
11:48 (cur | prev) +219 Korey Griffin talk contribs →‎siRNA transfection Procedure 6 well
 m   
11:48 (cur | prev) +219 Korey Griffin talk contribs →‎Verify transfection efficiency
     11:45  Yeo lab:Reprints‎‎ 2 changes history +304 [Yoonyeo‎ (2×)]
     
11:45 (cur | prev) -1 Yoonyeo talk contribs →‎2021
     
11:44 (cur | prev) +305 Yoonyeo talk contribs →‎2021
     10:58  Yeo lab‎‎ 2 changes history +232 [Yoonyeo‎ (2×)]
     
10:58 (cur | prev) +6 Yoonyeo talk contribs
     
10:57 (cur | prev) +226 Yoonyeo talk contribs
     10:58  Yeo lab:news archive diffhist +120 Yoonyeo talk contribs →‎2019
     09:53  Yeo lab:meetings‎‎ 2 changes history +6 [Yoonyeo‎ (2×)]
     
09:53 (cur | prev) +6 Yoonyeo talk contribs
     
09:53 (cur | prev) 0 Yoonyeo talk contribs
     09:42 User creation log User account Cfa 96 talk contribs was created by Yar talk contribs and password was sent by email ‎
     06:26  BioMicroCenter diffhist -65 Stuart S. Levine talk contribs

14 June 2021

13 June 2021

     20:01  RAVE:Install‎‎ 4 changes history +1,212 [Zhengjia‎ (4×)]
     
20:01 (cur | prev) +596 Zhengjia talk contribs →‎Install RAVE
     
18:56 (cur | prev) +14 Zhengjia talk contribs →‎Updating RAVE
     
16:55 (cur | prev) +560 Zhengjia talk contribs →‎Install RAVE
     
16:25 (cur | prev) +42 Zhengjia talk contribs →‎Install RAVE
     19:58  Hu:Publications‎‎ 2 changes history +239 [Hugangqing‎ (2×)]
     
19:58 (cur | prev) +236 Hugangqing talk contribs
     
04:05 (cur | prev) +3 Hugangqing talk contribs
     18:49  RAVE:Install prerequisites‎‎ 2 changes history +291 [Zhengjia‎ (2×)]
     
18:49 (cur | prev) -167 Zhengjia talk contribs →‎Prerequisites for Ubuntu
     
16:50 (cur | prev) +458 Zhengjia talk contribs →‎Prerequisites for MacOS

11 June 2021

     01:11  Experimental Anaesthesiology Antkowiak Lab:Publications‎‎ 2 changes history -603 [Vs.eckle‎ (2×)]
     
01:11 (cur | prev) -286 Vs.eckle talk contribs →‎2020
     
01:10 (cur | prev) -317 Vs.eckle talk contribs →‎2011
     01:08  Experimental Anaesthesiology Antkowiak Lab:Lab Members diffhist -43 Vs.eckle talk contribs
     00:30  (User creation log) [Yar‎ (3×)]
     
00:30 User account Wendyxv talk contribs was created by Yar talk contribs and password was sent by email ‎
     
00:30 User account Vs.eckle talk contribs was created by Yar talk contribs and password was sent by email ‎
     
00:30 User account Lucy26 talk contribs was created by Yar talk contribs and password was sent by email ‎

10 June 2021

</wikionly>

<nonwikionly> This page was created using Open Wetware.</nonwikionly>