Conor Keith Week 9

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Conor Keith 01:45, 16 March 2017 (EDT)

Purpose

  • The purpose of this assignment is to introduce DNA microarrays and learn how they can be used to analyze the behavior of genes.

Discovery Questions

  1. Newtrasgp.tif
  2. Colortable.jpg
  3. X and Y were transcribed relatively similarly. Both started at Black, then transcribed to Red, and ended at Green. Z remained relatively constant at Red.
  4. It is reasonable that most spots are initially yellow, as this indicates no change. At the initial point genes are not induced or repressed. Yellow is a mix of the other two possible colors, Red and Green.
  5. Over the course of the experiment TEF4 was repressed. TEF4 stimulates the binding of AA-tRNA to ribosomes. Lower rates of translation could be a response to a reduction in glucose
  6. TCA cycle genes are induced to support stress response under glucose starvation. The TCA cycle can produce ATP, NADH and FADH2 without the use of glucose. So TCA cycle genes are expressed to continue to produce energy in the absence of glucose.
  7. To ensure genes for enzymes are induced or repressed simultaneously the genome could cluster genes in the same promoter sequence. Gene clusters utilize microarray data to find groups of genes that share common attributes.
  8. The spots that represent glucose-repressed genes would appear red later in the experiment, as glucose starvation will lead to higher expression levels of these genes.
  9. Yap1p target spots would be red. Yap1p is resistant to glucose starvation, so they would continue to be expressed.
  10. If TUP1 is actually deleted the target spots would remain black throughout the whole experiment. IF Yap1p was overexpressed, the target spots would remain red throughout the experiment.

Presentation Model Redo

Acknowledgements

  • This individual journal entry was completed by me and not copied from any other source.

References

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