BioMicroCenter:Varioskan

HOME --

SEQUENCING --

LIBRARY PREP --

HIGH-THROUGHPUT --

COMPUTING --

OTHER TECHNOLOGY

PLATE READER

Instrument ThermoFisher Varioskan TYPE WALKUP - MIT only UNIT Per hour in 10' increments FORMAT 96w/394w FLUOROMETRY 6-1536w Excite: 200-1000nm Emission: 270-840nm Sensitivity: <0.4fmol fluorescein/w Dynamic Range: >6 decades LUMINOMETRY 6-384w spectral scan 360-670nm 270-840nm spectral scan Sensitivity: < amol ATP/w Dynamic Range: >7 decades PHOTOMETRY ABSORBANCE 6-384w Wavelength: 200-1000nm Sensitivity: 0.003Abs +/-2% Dynamic Range: 0-4Abs @450nm SIGNUP ilabs NEW USERS New users should request training by emailing biomicro@mit.edu DONATED BY Prof. Jeroen Saeij.

The Thermo Scientific Varioskan Flash Spectral Scanning Multimode Reader is a highly flexible plate reader capable of handling fluorometric (excitation/emission), luminometric (emission) and photometric (absorbance) measurements in 96+ well format. The Varioskan can handle a broad range of applications from reporter assays to FRET. The Varioskan is equipped with three on board fluid dispensers, incubator and shaker to allow for real time assays. Coupled with the Tecan EVO, the Varioskan should allow major improvements in high throughput experiment design. Currently the Varioskan is located in 68-316. The Varioskan supports: 3 onboard liquid dispensers 1ul – 10ml dispensing volumes Temperature control from ambient + 20C – 45C On board orbital shaker The varioskan is used routinely in the BioMicro Center to measure the concentration of Illumina libraries using SYBRgreen. The Varioskan will also be used to measure concentrations of oligos from Dr.Oligo. Suggested Assays: Apoptosis assays Ca2+ flux assays Cell proliferation Cellular assays Cytotoxicity and ADMETox DNA concentration ELISA/FIA/TRF-ELISA assays Enzyme kinetic studies Europium assays FRET assays TR-FRET assays BRET assays GPCR assays Ion channel assays Kinase assays Multilabel assays Reporter gene assays Signal transduction Tryptophan and tyrosine UV fluorescence