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BISC209: Enrichment: Difference between revisions
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→Enrichment media to isolate specific soil bacterial community members based on functional roles(Starting with either SOIL or SOIL EXTRACT)
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'''Identification:<BR>''' | '''Identification:<BR>''' | ||
These colonies may be slow growing, so check your plates every few days for up to 2 weeks. <li> | These colonies may be slow growing, so check your plates every few days for up to 2 weeks. <li> | ||
Look for hard, white, ridged colonies (little "volcanoes")characteristic of ''Streptomyces'' or "powdered sugar" colonies with an indentation of agar around the colony.<li> | Look for hard, white, ridged colonies (little "volcanoes")characteristic of ''Streptomyces'' or "powdered sugar" colonies with an indentation of agar around the colony.<li> | ||
Once you have relatively pure isolates without contaminating fungal growth, make a bacterial smear slide and Gram stain it(see Protocols for procedures)begin to examine the cellular morphology, arrangement, and cell wall structure of these bacteria. Look carefully for clear areas in the vegetative cells indicative of endospores. You will do an endospore stain in a later lab on any isolates that grow from this enrichment, but look carefully for this preliminary indication of endospores. | Once you have relatively pure isolates without contaminating fungal growth, make a bacterial smear slide and Gram stain it(see Protocols for procedures)begin to examine the cellular morphology, arrangement, and cell wall structure of these bacteria. Look carefully for clear areas in the vegetative cells indicative of endospores. You will do an endospore stain in a later lab on any isolates that grow from this enrichment, but look carefully for this preliminary indication of endospores. | ||
