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BISC209: Enrichment: Difference between revisions
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→Enrichment media to isolate specific soil bacterial community members based on functional roles(Starting with either SOIL or SOIL EXTRACT)
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'''Enrichment:'''<BR> | '''Enrichment:'''<BR> | ||
Your lab instructor will return (in LAB 2) the dried 1 g soil samples, that you weighed out in LAB 1.< | Your lab instructor will return (in LAB 2) the dried 1 g soil samples, that you weighed out in LAB 1.<li> | ||
Record the dried weight from the 3 samples in your lab notebooks<br>< | Record the dried weight from the 3 samples in your lab notebooks<br><li> | ||
Combine the 3 dried soil samples and then re-weigh to get 1 g of dried soil. Add it to 100 ml of dilute nutrient broth in a flask containing a magnetic stirrer. < | Combine the 3 dried soil samples and then re-weigh to get 1 g of dried soil. Add it to 100 ml of dilute nutrient broth in a flask containing a magnetic stirrer. <li> | ||
Agitate on a stir plate for 30 minutes.< | Agitate on a stir plate for 30 minutes.<li> | ||
Allow the large particles to settle to the bottom of the flask for 30 minutes.< | Allow the large particles to settle to the bottom of the flask for 30 minutes.<li> | ||
Soak a sterile cotton swab in the broth. < | Soak a sterile cotton swab in the broth. </li><BR> | ||
'''Isolation:'''<BR> | '''Isolation:'''<BR> | ||
