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BISC209: Enrichment: Difference between revisions
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→Enrichment media to isolate specific soil bacterial community members based on functional roles(Starting with either SOIL or SOIL EXTRACT)
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==Enrichment media to isolate specific soil bacterial community members based on functional roles(Starting with either SOIL or SOIL EXTRACT)== | ==Enrichment media to isolate specific soil bacterial community members based on functional roles(Starting with either SOIL or SOIL EXTRACT)== | ||
<font color="purple">'''Spore forming bacteria such as ''Bacillus'' and ''Streptomyces | <font color="purple">'''Spore forming bacteria such as members of genera ''Bacillus'' and of ''Streptomyces'' in the ''Actinomycetes'' family'' <BR> | ||
Glycerol Yeast medium'''</font color="purple"><BR> | Glycerol Yeast medium'''</font color="purple"><BR> | ||
Spore forming bacteria are highly resistant to environmental stresses and to disinfection procedures. Among these hardy bacteria are members of the genus Bacillus and the ''Actinomycetes'' group of bacteria (including the Streptomyces that are important sources of antibiotics. Actinomycetes often show a uniquely recognizable filamentous and/or leathery colonial morphology [http://www.microbelibrary.org/ASMOnly/details.asp?id=2566&Lang=&ISkip=20] that will help you find them. Most of the genera in this group produce a hyphal type of growth and form spores during their complex lifecycle. We will use oven dried soil on selective media to enrich and select for spore forming bacteria that can survive oven drying and high osmotic pressure in a selective medium. You should try to isolate and characterize several different appearing colonies from this medium. | Spore forming bacteria are highly resistant to environmental stresses and to disinfection procedures. Among these hardy bacteria are members of the genus Bacillus and the ''Actinomycetes'' group of bacteria (including the Streptomyces that are important sources of antibiotics. Actinomycetes often show a uniquely recognizable filamentous and/or leathery colonial morphology [http://www.microbelibrary.org/ASMOnly/details.asp?id=2566&Lang=&ISkip=20] that will help you find them. Most of the genera in this group produce a hyphal type of growth and form spores during their complex lifecycle. We will use oven dried soil on selective media to enrich and select for spore forming bacteria that can survive oven drying and high osmotic pressure in a selective medium. You should try to isolate and characterize several different appearing colonies from this medium. Do some research on the web to find images of macroscopic colonies and microscopic bacteria from these groups so you will recognize them if you find them. | ||
<UL><LI> | <UL><LI> | ||
'''Glycerol Yeast Extract Agar''': a | '''Glycerol Yeast Extract Agar''': a selective medium to enrich for many of the spore forming, antibiotic producing bacteria in the ''Actinomycetes'', Bacillus, and other groups of Gram positive spore formers.<UL><LI> | ||
'''Enrichment:'''<BR> | '''Enrichment:'''<BR> | ||
