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BioMicroCenter:Microarrays: Difference between revisions
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== BioMicro Center Services == | == BioMicro Center Services == | ||
>> THIS PAGE IS CURRENTLY BEING EDITED << | |||
We are currently set up to primarily handle expression analysis. Our service includes: | We are currently set up to primarily handle expression analysis. Our service includes: | ||
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We also have discounts with different vendors and can help you purchase your arrays. | We also have discounts with different vendors and can help you purchase your arrays. | ||
<br> | <br> | ||
=== ARRAY SELECTION === | |||
There are several considerations to take into account in selecting your microarray. We provide support for multiple array platforms from two different manufacturers (Agilent and Affymetrix). Additionally, multiple labeling methods are available for each platform. Each labeling method has different sample requirements and multiple labeling methods should NOT be combined in doing post-hybridization analysis. Below are a list of different sample types and our recommended labeling methodologies: | |||
=== SAMPLE TYPES === | === SAMPLE TYPES === | ||
The BioMicro Center has extensive experience handling samples for expression analysis. | The BioMicro Center has extensive experience handling samples for expression analysis. | ||
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|- | |- | ||
| 3' Array (std) | | 3' Array (std) | ||
| 50ng / 100pg | | 50ng / ''100pg '' | ||
| 6 μL | | 6 μL | ||
| labeling using NuGEN for Affy arrays. | | labeling using NuGEN for Affy arrays. | ||
|- | |- | ||
| Exon Array | | Exon Array | ||
| 50ng / 100pg | | 50ng / ''100pg'' | ||
| 6 μL | | 6 μL | ||
| labeling using NuGEN for Affy arrays | | labeling using NuGEN for Affy arrays | ||
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| colspan="7" | '''AFFYMETRIX PROCESSING''' | | colspan="7" | '''AFFYMETRIX PROCESSING''' | ||
|- | |- | ||
| Affy 3' Processing || $260 || $430 || $645 || per array || Using NuGEN sample labeling kit | | Affy 3' Processing || $260 || $430 || $645 || per array || Using NuGEN or AFFY sample labeling kit | ||
|- | |- | ||
| Affy Exon Processing || $320 || $535 || $800 || per array || Using NuGEN sample labeling kit | | Affy Exon Processing || $320 || $535 || $800 || per array || Using NuGEN or AFFTY sample labeling kit | ||
|- | |- | ||
| Affy Prokaryote || $250 || $430 || $645 || per array || Using Ambion sample labeling kit | | Affy Prokaryote || $250 || $430 || $645 || per array || Using Ambion sample labeling kit | ||
|- | |- | ||
| Fluidics Station and Scan || $130 ||$130 || | | Fluidics Station and Scan || $130 ||$130 || $180 || per array || Users must be trained, please coordinate with Manlin | ||
|- | |- | ||
| colspan="7" | '''AGILENT PROCESSING''' | | colspan="7" | '''AGILENT PROCESSING''' | ||
