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BioMicroCenter:Sequencing: Difference between revisions
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→LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE
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Illumina Sequencing is performed on a lane-by-lane basis instead of for a specific number of reads. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation|Illumina Library]] types as well as handle custom sequencing applications. <BR><BR> | Illumina Sequencing is performed on a lane-by-lane basis instead of for a specific number of reads. This enables us to support a very broad spectrum of [[BioMicroCenter:Illumina_Library_Preparation|Illumina Library]] types as well as handle custom sequencing applications. <BR><BR> | ||
== LONG READ SEQUENCING / PACBIO and OXFORD NANOPORE == | == LONG READ SEQUENCING / [[BioMicroCenter:PacBio|PACBIO]] and OXFORD NANOPORE == | ||
The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: Pacific Biosciences | The BioMicro Center has made significant investments in long read sequencers. The Center has access to two separate platforms: [[BioMicroCenter:PacBio|Pacific Biosciences Sequel]] and Oxford Nanopore Minion.<BR><BR> | ||
Pacific BioSciences sequencing is performed on the Sequel with | Pacific BioSciences sequencing is performed on the Sequel with v3 chemistry. PacBio requests can be made through iLabs.<BR><BR> | ||
Oxford Nanopore sequencing is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. ONT minion runs are done on a custom basis only. Please email biomicro@mit.edu if you are interested in using the Minion. <BR><BR> | Oxford Nanopore sequencing is still a new technology in the core but we are developing expertise that may be useful to researchers on campus. ONT minion runs are done on a custom basis only. Please email biomicro@mit.edu if you are interested in using the Minion. <BR><BR> | ||
