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The FEMTO Pulse is a brand new instrument that provides a remarkable upgrade over the existing [[BioMicroCenter:Advanced_analytical_Fragment_analyzer|AATI Fragment Analyzer] and Agilent BioAnalyzer technologies that have been available for many years. While still an instrument to analyze the lengths of nucleic acids, the FEMTO Pulse adds a pulse filed generator to allow the separation and characterization of significantly longer molecules, out to 200,000 base pairs. Additionally, the FEMTO pulse has significantly improved optics that allow approximately two orders of magnitude of additional sensitivity. Where the AATI fragment analyzer bottoms out a sensitivity of 2-3pg/ul, the FEMTO pulse has nearly 100x improved sensitivity which may enable this instrument to directly observe molecular reactions without the need for amplification or modification with radioactive nucleotides.
The FEMTO Pulse runs 12 samples at a time using 2ul of material. Each run takes ~[unknown] minutes to run. It is *not* a replacement for the Fragment Analyzer and is optimized for very low inputs or very long samples.
The FEMTO Pulse was donated to the BioMicro Center by MIT CEHS.