User contributions for Skim

22 August 2007

• 21:4121:41, 22 August 2007 diff hist +446 IGEM:MIT/2007/Notebook/2007-8-22 →Agenda

9 August 2007

• 21:3821:38, 9 August 2007 diff hist −1 IGEM:MIT/2007/Notebook/2007-8-9 →Accomplished Today current
• 21:3721:37, 9 August 2007 diff hist +7 IGEM:MIT/2007/Notebook/2007-8-9 →Miniprepped Standard Assembly LCs
• 21:3721:37, 9 August 2007 diff hist +4 IGEM:MIT/2007/Notebook/2007-8-9 →Miniprepped Standard Assembly LCs
• 21:3621:36, 9 August 2007 diff hist +908 N IGEM:MIT/2007/Notebook/2007-8-9 New page: ===Accomplished Today=== ==Miniprepped Standard Assembly LCs== *12 tubes, 4 each of CPX/B0014, I14032/B0034 and R0051/B0034, all in DH5alpha *NOTE: Elution with Tris EDTA, not water *Nanod...

7 August 2007

• 20:0920:09, 7 August 2007 diff hist +175 IGEM:MIT/2007/Notebook/2007-8-7 No edit summary current

30 July 2007

• 19:5819:58, 30 July 2007 diff hist +8 IGEM:MIT/2007/Notebook/2007-7-30 →Live Cell Immunoassay (Eric)
• 18:3118:31, 30 July 2007 diff hist +31 IGEM:MIT/2007/Notebook/2007-7-30 →Live Cell Immunoassay (Eric)
• 18:3018:30, 30 July 2007 diff hist +665 IGEM:MIT/2007/Notebook/2007-7-30 No edit summary

24 July 2007

• 20:4420:44, 24 July 2007 diff hist +537 IGEM:MIT/2007/Notebook/2007-7-24 →AGENDA
• 20:3720:37, 24 July 2007 diff hist +685 IGEM:MIT/2007/Notebook/2007-7-24 →AGENDA
• 13:3913:39, 24 July 2007 diff hist +548 N IGEM:MIT/2007/Notebook/2007-7-24 New page: ==AGENDA== *Plate the Mer/I13500 3A transformation after overnight ligation (and grow) *Check if sequence results are available from Monday (length was correct) *Second polystyrene assay (...

23 July 2007

• 19:3519:35, 23 July 2007 diff hist +19 IGEM:MIT/2007/Notebook/2007-7-23 →3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3 (SK, JH, BH)
• 19:3419:34, 23 July 2007 diff hist 0 IGEM:MIT/2007/Notebook/2007-7-23 →Agenda
• 19:3319:33, 23 July 2007 diff hist −1 IGEM:MIT/2007/Notebook/2007-7-23 →Agenda
• 19:3219:32, 23 July 2007 diff hist +37 IGEM:MIT/2007/Notebook/2007-7-23 →3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3
• 19:3019:30, 23 July 2007 diff hist +319 IGEM:MIT/2007/Notebook/2007-7-23 →3A Ligation of Mer promoter+Mer regulatory protein (pTO40) and RBS+GFP (I13500) into pSB1AT3
• 19:2619:26, 23 July 2007 diff hist +800 IGEM:MIT/2007/Notebook/2007-7-23 →Digestion of I13500

20 July 2007

• 19:2919:29, 20 July 2007 diff hist +45 IGEM:MIT/2007/Notebook/2007-7-20 →Agenda current
• 19:2819:28, 20 July 2007 diff hist +13 IGEM:MIT/2007/Notebook/2007-7-20 →Agenda
• 19:2819:28, 20 July 2007 diff hist +227 IGEM:MIT/2007/Notebook/2007-7-20 →Agenda

19 July 2007

• 15:4515:45, 19 July 2007 diff hist +398 IGEM:MIT/2007/Notebook/2007-7-19 →Agenda for Thursday, 7/19

18 July 2007

• 19:3219:32, 18 July 2007 diff hist +43 IGEM:MIT/2007/Notebook/2007-7-19 →Agenda for Thursday, 7/19
• 19:3019:30, 18 July 2007 diff hist +6 IGEM:MIT/2007/Notebook/2007-7-19 →Agenda for Thursday, 7/19
• 19:3019:30, 18 July 2007 diff hist +61 N IGEM:MIT/2007/Notebook/2007-7-19 New page: ===Agenda for Thursday, 7/19=== *Plating (K+/A+)? *AK/AL list
• 16:4816:48, 18 July 2007 diff hist 0 IGEM:MIT/2007/Notebook/2007-7-18 →Agenda
• 16:4716:47, 18 July 2007 diff hist +385 IGEM:MIT/2007/Notebook/2007-7-18 →Agenda

17 July 2007

• 16:0816:08, 17 July 2007 diff hist +419 IGEM:MIT/2007/Notebook/2007-7-17 →Digestion of (F2620 and B0034) for 3A

15 July 2007

• 06:4406:44, 15 July 2007 diff hist +1 IGEM:MIT/2007/Notebook/2007-7-14 →Weekend Update
• 06:4306:43, 15 July 2007 diff hist +28 IGEM:MIT/2007/Notebook/2007-7-14 →Late Friday (7/13) Afternoon Update
• 06:4306:43, 15 July 2007 diff hist +1,908 N IGEM:MIT/2007/Notebook/2007-7-14 New page: ===Late Friday (7/13) Afternoon Update=== *BH/SK put dry pT040 (?) plates in warm room (for AK and AL) *Tom stopped by -- we have a BUNCH of chemically competent cells in the -80 degree fr...

11 July 2007

• 18:0418:04, 11 July 2007 diff hist +443 IGEM:MIT/2007/Room Reservations No edit summary

9 July 2007

• 17:4817:48, 9 July 2007 diff hist +1 IGEM:MIT/2007/Notebook/2007-7-3 →Incubated plates of DH5a current
• 15:0415:04, 9 July 2007 diff hist +4 IGEM:MIT/2007/Notebook/2007-7-3 →Make LB+KAN agar plates

7 July 2007

• 21:4321:43, 7 July 2007 diff hist +458 IGEM:MIT/2007/Notebook/2007-7-7 →Checked Plates 5 PM current

2 July 2007

• 21:3521:35, 2 July 2007 diff hist +37 IGEM:MIT/2007/Notebook/2007-7-3 →Agenda for July 3
• 21:3521:35, 2 July 2007 diff hist +41 IGEM:MIT/2007/Notebook/2007-7-3 →Agenda for July 3
• 21:3421:34, 2 July 2007 diff hist +140 N IGEM:MIT/2007/Notebook/2007-7-3 New page: ===Agenda for July 3=== *Call Geneart *Results of Alex/Diti PCR? *For metals: RBS and promoter? (clarify!) *Barry's promoter -- obtainment?
• 03:1903:19, 2 July 2007 diff hist +27 IGEM:MIT/2007/Notebook/2007-7-2 →Agenda: Monday, 7/2

29 June 2007

• 20:2520:25, 29 June 2007 diff hist +39 N IGEM:MIT/2007/Notebook/2007-7-2 New page: ===Agenda: Monday, 7/2=== *Order stuff!
• 05:3005:30, 29 June 2007 diff hist +507 N IGEM:MIT/2007/Notebook/2007-6-29 New page: ===Agenda for Friday, June 29=== *Morning **pBAD pickup **Is OmpA crucial to bacterial cell survival? **Finalize oligos (restriction sites) **Research "red team" stuff -- ion exchange **Br...

25 June 2007

• 19:1719:17, 25 June 2007 diff hist +412 N Talk:IGEM:MIT/2007/Notebook/2007-6-25 New page: ===CHECKLIST for Monday, 6/25=== *Reorganize wiki *CPX/OmpC inserts **Polystyrene 17 **Polystyrene 18 (Belcher?) **Polypyrrole *Check sequences for CPX *Call Brian/grads/Eric check inserts... current
• 19:0519:05, 25 June 2007 diff hist +686 N IGEM:MIT/2007/Notebook/2007-6-25 New page: ===USING SEQUENCHER (Demo)=== *Copy desired gene sequence (to check) *Sequence tab **Create New Sequence **Give name **Paste sequence *View tab **Translation – different reading frame...

20 June 2007

• 14:5014:50, 20 June 2007 diff hist +742 IGEM:MIT/2007/Ideas3 →Mussel Protein

18 June 2007

• 04:4704:47, 18 June 2007 diff hist +1 IGEM:MIT/2007/Notebook/2007-6-17 →Notes from Meeting at 8 pm current
• 04:4604:46, 18 June 2007 diff hist +2,971 IGEM:MIT/2007/Notebook/2007-6-17 No edit summary

12 June 2007

• 03:1103:11, 12 June 2007 diff hist 0 IGEM:MIT/2007/Notebook/2007-6-11 →Making Plates
• 03:1103:11, 12 June 2007 diff hist −2 IGEM:MIT/2007/Notebook/2007-6-11 →Transforming DNA plasmids into bacterial colonies on plates
• 03:0903:09, 12 June 2007 diff hist −2 IGEM:MIT/2007/Notebook/2007-6-11 →General Lab Do s and Don’t s
• 03:0803:08, 12 June 2007 diff hist −10 IGEM:MIT/2007/Notebook/2007-6-11 →General Lab Do s and Don’t s