IGEM:MIT/2007/Notebook/2007-6-29
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Agenda for Friday, June 29
- Morning
- pBAD pickup
- Is OmpA crucial to bacterial cell survival?
- Finalize oligos (restriction sites)
- Research "red team" stuff -- ion exchange
- Brian -- stainless steel, nylon binding?
- Check on FhuA and pSB3K3 colonies
- Afternoon
- Look for metal inducible promoters (Mer) to fuse to GFP/something FP/whatever
- Order above oligo
- Find/finalize vector (pBAD33?)
- Have everything ordered by the end of today!!
- Question to think about: metal ion diffusion into the cell or binding at the surface?
pSB3K3
- 9AM - Good news, all of the colonies we picked last night are cloudy today! :D
