User contributions for David Roche

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A user with 579 edits. Account created on 6 July 2009.

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14:4014:40, 4 September 2009 diff hist 0 File:II09 FullSystem.png uploaded a new version of "Image:II09 FullSystem.png" current
14:3814:38, 4 September 2009 diff hist 0 File:II09 FullSystem.png uploaded a new version of "Image:II09 FullSystem.png"
14:3414:34, 4 September 2009 diff hist 0 N File:II09 FullSystem.png No edit summary
14:3414:34, 4 September 2009 diff hist +50 N IGEM:IMPERIAL/2009/Full System New page: === Full System === Image:II09_FullSystem.png current
14:3314:33, 4 September 2009 diff hist +2 IGEM:IMPERIAL/2009/4Sep →Agenda
14:3314:33, 4 September 2009 diff hist +35 IGEM:IMPERIAL/2009/4Sep →Agenda
13:1713:17, 4 September 2009 diff hist +7 IGEM:IMPERIAL/2009/4Sep →Agenda
13:1713:17, 4 September 2009 diff hist +51 IGEM:IMPERIAL/2009/4Sep →Agenda
12:3612:36, 4 September 2009 diff hist 0 N File:II09 M1a C3.jpg No edit summary current
12:3512:35, 4 September 2009 diff hist +27 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 1 current
12:3512:35, 4 September 2009 diff hist +4 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 0
12:3412:34, 4 September 2009 diff hist −1 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 0
12:3412:34, 4 September 2009 diff hist 0 N File:II09 M0 C2.jpg No edit summary current
12:3312:33, 4 September 2009 diff hist +37 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated →Module 0
12:3112:31, 4 September 2009 diff hist +27 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated No edit summary
12:2912:29, 4 September 2009 diff hist +24 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated No edit summary
12:2812:28, 4 September 2009 diff hist 0 N File:II09 C1.jpg No edit summary current
12:2812:28, 4 September 2009 diff hist −1 IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated No edit summary
12:2412:24, 4 September 2009 diff hist 0 N File:II09 M0a.png No edit summary current
12:2412:24, 4 September 2009 diff hist +50 N IGEM:IMPERIAL/2009/Assays Protocols/Testing Constructs Updated New page: Testing Constructs Updated Image:II09_M0a.png

15:4415:44, 3 September 2009 diff hist 0 N File:II09 logoidea1.png No edit summary current
15:4415:44, 3 September 2009 diff hist +28 N IGEM:IMPERIAL/2009/Wiki Logo/i New page: Image:II09_logoidea1.png
10:3110:31, 3 September 2009 diff hist 0 N File:II09 Temp2.png No edit summary current
10:3110:31, 3 September 2009 diff hist +24 N IGEM:IMPERIAL/2009/Wiki Templates/ii New page: Image:II09_Temp2.png current
10:3010:30, 3 September 2009 diff hist 0 N File:II09 Temp1.png No edit summary current
10:3010:30, 3 September 2009 diff hist +4 IGEM:IMPERIAL/2009/Wiki Templates/i No edit summary current
10:2910:29, 3 September 2009 diff hist +20 N IGEM:IMPERIAL/2009/Wiki Templates/i New page: Image:II09_Temp1

09:5509:55, 2 September 2009 diff hist +203 N Calendar:ImperialWetLabNotebook2009iGEM/2009-9-2 New page: ~~~~ <br> AM : Ran a gel with the WaaL pcr products that were digested overnight. 20ul against the ladder <br> Updated wiki and BioBrick registry

12:4012:40, 1 September 2009 diff hist +41 IGEM:IMPERIAL/2009/M2 →Cloning strategy (assembly) current

14:5314:53, 24 August 2009 diff hist +19 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Diagram current
14:5214:52, 24 August 2009 diff hist +125 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Diagram
14:4914:49, 24 August 2009 diff hist +211 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Model
14:4114:41, 24 August 2009 diff hist +240 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer2 →Diagram
14:2114:21, 24 August 2009 diff hist +8 m IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer1 →Operations current
14:2114:21, 24 August 2009 diff hist +184 IGEM:IMPERIAL/2009/Encapsulation/Modelling/Timer1 →Operations
13:2813:28, 24 August 2009 diff hist −4 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-24 →WaaL ligase PCR current
13:2813:28, 24 August 2009 diff hist +454 N Calendar:ImperialWetLabNotebook2009iGEM/2009-8-24 New page: ~~~~<br> ===WaaL ligase PCR === <br> <br> * Used Barnes buffer instead of Pfu Ultra Buffer * PCR taken at 48'C, 50'C and 52'C with the negative at 48'C * Successful PCR (at last) at all 3...

14:3714:37, 21 August 2009 diff hist +1 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 →Gel Extraction of OtsA, RcsB, Dam current
14:3614:36, 21 August 2009 diff hist −49 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 →Gel Extraction of OtsA, RcsB, Dam
14:3514:35, 21 August 2009 diff hist +389 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 →Gel Extraction of OtsA, RcsB, Dam
14:3114:31, 21 August 2009 diff hist +66 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 No edit summary
14:3014:30, 21 August 2009 diff hist +828 N Calendar:ImperialWetLabNotebook2009iGEM/2009-8-21 New page: === WaaL Ligase PCR === <br> <i> 2nd attempt, as it did not work yesterday </i> <br> PCR was using:<br> * TOP10 e-coli genome. * Pfu II Ultra Enzyme * Pfu II Ultra Buffer * dNTPs, fwd and ...

16:2716:27, 19 August 2009 diff hist +1 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
16:2616:26, 19 August 2009 diff hist +107 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
16:1716:17, 19 August 2009 diff hist +66 IGEM:IMPERIAL/2009/M2/Assays →Assay 3.2: Trehalose Functionality current
16:0816:08, 19 August 2009 diff hist +437 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →Results of Gel
16:0516:05, 19 August 2009 diff hist +823 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
15:5615:56, 19 August 2009 diff hist +46 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
15:5515:55, 19 August 2009 diff hist +281 Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 →PAH Site-Directed Mutagenesis
15:5315:53, 19 August 2009 diff hist +411 N Calendar:ImperialWetLabNotebook2009iGEM/2009-8-19 New page: ~~~~ === PAH Site-Directed Mutagenesis === <i> There are 2 sites in PAH that require site directed mutagenesis (one XbaI site and one PstI site). We chose to start with the XbaI site, ...

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