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David Roche 09:28, 24 August 2009 (EDT)

WaaL ligase PCR

  • Used Barnes buffer instead of Pfu Ultra Buffer
  • PCR taken at 48'C, 50'C and 52'C with the negative at 48'C
  • Successful PCR (at last!) at all 3 temperatures. We get the same unspecific band as we have been getting previously (perhaps the sterile water is not so sterile??).
  • will run a restriction digest on the 52'C PCR product overnight, later today.