OpenWetWare:Community development

Welcome to the discussion area for planning community development on OpenWetWare.

How you can help

We are in the process of setting up this page, content that should be aggregated here currently lives on several other pages:

• OpenWetWare steering committee/Promotional material ideas, OpenWetWare:Presentations and OpenWetWare:Poster

Please feel free to move this content into this space in a more organized manner, in particular discussions should be moved to relevant talk pages, and we should start specifying subarticles.

Active Discussions

• OpenWetWare:Community development/Outreach - help promote OWW at your institution!
• OpenWetWare:Steering committee/What positions would be useful?
• OpenWetWare:Community development/Non-English versions
• OpenWetWare talk:Community development/Highlights
• Removing Inactive Sites
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Subarticles

• OpenWetWare:Community development/Advertising
• OpenWetWare:Community development/Posters
• OpenWetWare:Community development/Presentations

Recent Changes in Community Development

List of abbreviations:

N

This edit created a new page (also see list of new pages)

m

This is a minor edit

b

This edit was performed by a bot

(±123)

The page size changed by this number of bytes

4 June 2023

m   07:44

User:Richard J. Abbott‎ diffhist +8‎ Richard J. Abbott talk contribs (→‎Publications)

3 June 2023

			23:44		Lee:JC‎‎ 6 changes history +26‎ [Wooin Lee‎ (6×)]
					23:44 (cur | prev) +9‎ Wooin Lee talk contribs (→‎Schedule)
					23:23 (cur | prev) −10‎ Wooin Lee talk contribs (→‎Schedule)
					23:22 (cur | prev) +6‎ Wooin Lee talk contribs (→‎Schedule)
					23:21 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					23:01 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					22:53 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)

1 June 2023

			23:23		Lee:Lab Members‎‎ 4 changes history +169‎ [Wooin Lee‎ (4×)]
					23:23 (cur | prev) +164‎ Wooin Lee talk contribs (→‎Research rotation/internship students (past))
					23:21 (cur | prev) +26‎ Wooin Lee talk contribs (→‎Graduate students)
					23:20 (cur | prev) +31‎ Wooin Lee talk contribs (→‎Alumni)
					23:19 (cur | prev) −52‎ Wooin Lee talk contribs (→‎Graduate students)

17:46

BioMicroCenter:Pricing‎ diffhist −79‎ Stuart S. Levine talk contribs (→‎SHORT READ LIBRARY - RNA)

			09:04		(Upload log)‎ [Elizabeth Suesca‎ (2×)]
					09:04 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png ‎
					08:25 Elizabeth Suesca talk contribs uploaded File:Acetato.png ‎

31 May 2023

N    14:39

UA Biophysics:Protocols:Carotenoid Extration ESP‎ diffhist +423‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo")

14:24

UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer‎ diffhist +54‎ Elizabeth Suesca talk contribs

14:23

UA Biophysics:Protocols‎ diffhist −7‎ Elizabeth Suesca talk contribs (→‎BUFFERS)

			N    14:22		UA Biophysics:Protocols:Elution Buffer‎‎ 2 changes history +1,055‎ [Elizabeth Suesca‎ (2×)]
					14:22 (cur | prev) +467‎ Elizabeth Suesca talk contribs
			N		14:07 (cur | prev) +588‎ Elizabeth Suesca talk contribs (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...")

			N    14:03		UA Biophysics:Protocols:Calcein ESP‎‎ 3 changes history +1,249‎ [Elizabeth Suesca‎ (3×)]
					14:03 (cur | prev) +43‎ Elizabeth Suesca talk contribs (→‎Procedimiento:)
					14:02 (cur | prev) +63‎ Elizabeth Suesca talk contribs
			N		14:00 (cur | prev) +1,143‎ Elizabeth Suesca talk contribs (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...")

12:48

BioMicroCenter:QC‎ diffhist −421‎ Stuart S. Levine talk contribs (→‎AGILENT BIOANALYZER 2100)

			08:34		UA Biophysics:Protocols:Calcein‎‎ 2 changes history −751‎ [Elizabeth Suesca‎ (2×)]
					08:34 (cur | prev) +53‎ Elizabeth Suesca talk contribs
					08:33 (cur | prev) −804‎ Elizabeth Suesca talk contribs (Replaced content with " Calcein_ESP<br>") Tag: Replaced

N    08:31

UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP‎ diffhist +1,206‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")

06:42

UA Biophysics:Protocols:Lipid Extraction‎ diffhist −9‎ Elizabeth Suesca talk contribs

30 May 2023

23:52

Hexlab‎ diffhist +440‎ Ximiao He talk contribs

Community Update Email Messages

List of abbreviations:

N

This edit created a new page (also see list of new pages)

m

This is a minor edit

b

This edit was performed by a bot

(±123)

The page size changed by this number of bytes

4 June 2023

m   07:44

User:Richard J. Abbott‎ diffhist +8‎ Richard J. Abbott talk contribs (→‎Publications)

3 June 2023

			23:44		Lee:JC‎‎ 6 changes history +26‎ [Wooin Lee‎ (6×)]
					23:44 (cur | prev) +9‎ Wooin Lee talk contribs (→‎Schedule)
					23:23 (cur | prev) −10‎ Wooin Lee talk contribs (→‎Schedule)
					23:22 (cur | prev) +6‎ Wooin Lee talk contribs (→‎Schedule)
					23:21 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					23:01 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)
					22:53 (cur | prev) +7‎ Wooin Lee talk contribs (→‎Schedule)

1 June 2023

			23:23		Lee:Lab Members‎‎ 4 changes history +169‎ [Wooin Lee‎ (4×)]
					23:23 (cur | prev) +164‎ Wooin Lee talk contribs (→‎Research rotation/internship students (past))
					23:21 (cur | prev) +26‎ Wooin Lee talk contribs (→‎Graduate students)
					23:20 (cur | prev) +31‎ Wooin Lee talk contribs (→‎Alumni)
					23:19 (cur | prev) −52‎ Wooin Lee talk contribs (→‎Graduate students)

17:46

BioMicroCenter:Pricing‎ diffhist −79‎ Stuart S. Levine talk contribs (→‎SHORT READ LIBRARY - RNA)

			09:04		(Upload log)‎ [Elizabeth Suesca‎ (2×)]
					09:04 Elizabeth Suesca talk contribs uploaded File:Cloroformo.png ‎
					08:25 Elizabeth Suesca talk contribs uploaded File:Acetato.png ‎

31 May 2023

N    14:39

UA Biophysics:Protocols:Carotenoid Extration ESP‎ diffhist +423‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 6 gramos de célula== * Preparar centrífuga a 4 grados * 200 ml Metanol. Opcional: Agregar 2,6-Di-tert-butyl-4-methylphenol 0.1 % w/V (250 mg/250 ml) Para la extracción de carotenos, ya que los protegen de oxidación * 500 NaCl 1,7 M (99.348 g/1 L) * 200 Acetato de etilo (Mejor si esta destilado ya que tiende a ser muy hidrófilo) * Opcional Na2SO4 anhidro (1 g/ 500 ml de solución) * Cloroformo")

14:24

UA Biophysics:Protocols:K2HPO4 KH2PO4 Buffer‎ diffhist +54‎ Elizabeth Suesca talk contribs

14:23

UA Biophysics:Protocols‎ diffhist −7‎ Elizabeth Suesca talk contribs (→‎BUFFERS)

			N    14:22		UA Biophysics:Protocols:Elution Buffer‎‎ 2 changes history +1,055‎ [Elizabeth Suesca‎ (2×)]
					14:22 (cur | prev) +467‎ Elizabeth Suesca talk contribs
			N		14:07 (cur | prev) +588‎ Elizabeth Suesca talk contribs (Created page with " '''Buffer HEPES: 2 L a 400 mOsM pH 7.4 ==Materiales== MW (g/mol) [] mM mOsM M HEPES 238.30 20 20 9.532 g NaCl 58.44 170 340 19.869 g NaOH (1 M) 8 8 16 ml NaCl 16 32 1.870 g Colocar 1.6 litros de agua deionizada en la botella Pesar el HEPES y el NaCl, diluir en los 1,6 L de dH2O Ajustar pH usando NaOH a 7.4. Calcular la osmolaridad y completar los 400 mOsm con NaCl Completar los dos litros UA Biop...")

			N    14:03		UA Biophysics:Protocols:Calcein ESP‎‎ 3 changes history +1,249‎ [Elizabeth Suesca‎ (3×)]
					14:03 (cur | prev) +43‎ Elizabeth Suesca talk contribs (→‎Procedimiento:)
					14:02 (cur | prev) +63‎ Elizabeth Suesca talk contribs
			N		14:00 (cur | prev) +1,143‎ Elizabeth Suesca talk contribs (Created page with "'''Calceina: 400 mOsM, 50 mM, 100 ml, pH 7.4 ''' ==Materiales: == * Probeta 100 ml * 30 ml de NaOH (1M) * HCl (1M) * 3,113 g de calceina * 40 ml Buffer H: ** 5 ml de EDTA 0.2 mM (Para que quede a 0.01 mM en la solución final) ** HEPES 238.3 mg (para que quede a 10 mM en la solución final). ==Procedimiento: == # En la probeta medir 20 ml de NaOH 1M y colocar agitador # Disolver en el NaOH 3,113 g de calceina # Agregar los 40 ml del Buffer H a la calceina # A...")

12:48

BioMicroCenter:QC‎ diffhist −421‎ Stuart S. Levine talk contribs (→‎AGILENT BIOANALYZER 2100)

			08:34		UA Biophysics:Protocols:Calcein‎‎ 2 changes history −751‎ [Elizabeth Suesca‎ (2×)]
					08:34 (cur | prev) +53‎ Elizabeth Suesca talk contribs
					08:33 (cur | prev) −804‎ Elizabeth Suesca talk contribs (Replaced content with " Calcein_ESP<br>") Tag: Replaced

N    08:31

UA Biophysics:Protocols:Bacteria for Lipid Extraction ESP‎ diffhist +1,206‎ Elizabeth Suesca talk contribs (Created page with "==Materiales para 1.4 g de célula== * Plato con SA401, máximo de un mes desde la recuperación <br> * Frasco de vidrio con 10 ml de LB<br> * 2 L de LB repartidos en 13 Erlenmeyer de 500 ml<br> ==Procedimiento== '''Dia 1''' 4:00 pm ON de SA401: una colonia en el frasco con 10 ml de LB. <br> '''Dia 2''' 8:30 am Dilución de las células: 10 ul en cada Erlenmeyer<br> '''Dia 3''' 8:30 am Concentrar la muestra <br> * Prepara la centrífuga a 4°C. <br> * Refrigerar lo...")

06:42

UA Biophysics:Protocols:Lipid Extraction‎ diffhist −9‎ Elizabeth Suesca talk contribs

30 May 2023

23:52

Hexlab‎ diffhist +440‎ Ximiao He talk contribs