Non: Week 14

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Course Work
Assignments Journal Pages Shared Class Journal Pages
BIOL368/S20:Week 1 BIOL368/S20:Class Journal Week 1
BIOL368/S20:Week 2 Non: Week 2 BIOL368/S20:Class Journal Week 2
BIOL368/S20:Week 3 Non: Week 3 BIOL368/S20:Class Journal Week 3
BIOL368/S20:Week 4 Non: Week 4 BIOL368/S20:Class Journal Week 4
BIOL368/S20:Week 5 Non: Week 5 BIOL368/S20:Class Journal Week 5
BIOL368/S20:Week 6 Non: Week 6 BIOL368/S20:Class Journal Week 6
BIOL368/S20:Week 8 Non: Week 8 BIOL368/S20:Class Journal Week 8
BIOL368/S20:Week 10 Non: Week 10 BIOL368/S20:Class Journal Week 10
BIOL368/S20:Week 11 Non: Week 11 BIOL368/S20:Class Journal Week 11
BIOL368/S20:Week 13 Non: Week 13 BIOL368/S20:Class Journal Week 13
BIOL368/S20:Week 14 Non: Week 14 BIOL368/S20:Class Journal Week 14


The purpose of this week's lab was to research the relationship between SARS-CoV-1/SARS-CoV-2 and the ACE2 receptor of different species. Specifically, our group chose to study a random assortment of primates because of their close relationship to humans.

Combined Methods/Results

We settled on analyzing the structure and function relationship between the ACE2 receptors of primates and SARS-CoV-2 (and SARS-CoV-1 for additional background). We chose primates at random, as long as they had a ACE2 receptor gene. The fifteen we chose were:

  • Pongo abelii (Sumatran orangutan)
  • Macaca nemestrina (Southern pig-tailed macaque)
  • Pan troglodytes (Chimpanzee)
  • Tarsius syrichta (Philippine tarsier)
  • Nomascus leucogenys (Northern white-cheeked gibbon)
  • Propithecus coquereli (Coquerel's sifaka)
  • Chlorocebus sabaeus (Green monkey)
  • Saimiri boliviensis boliviensis (Black-capped squirrel monkey)
  • Macaca fascicularis (Crab-eating macaque)
  • Aotus nancymaae (Nancy Ma's night monkey)
  • Pan paniscus (Bonobo)
  • Rhinopithecus bieti (Black snub-nosed monkey)
  • Papio anubis (Olive baboon)
  • Macaca mulatta (Rhesus macaque)
  • Rhinopithecus roxellana (Golden snub-nosed monkey)

I specifically worked on sequence analysis of the fifteen primates and human ACE2.

  • I clustally aligned on the sixteen sequences (15 primates + homo sapiens) and imported them into Excel.
  • I highlighted amino acid residues based on their polarity.
  • I determined the consensus between the 16 sequences and calculated the percentage of residues that corresponded with the consensus.
  • I looked at 12 amino acid residue locations identified by Melin et al. (2020) that were deemed important to SARS-CoV-2 binding to ACE2 in humans.
  • I calculated the number of residues that were conserved/semi-conserved/non-conserved at these 12 locations for each of the primates. This chart can be seen in the final presentation.


Scientific Conclusion

We found that many primates would be susceptible to SARS-CoV-2 based on their ACE2 sequence similarity to humans. These primates shared the same binding residues in the ACE2 receptor that allow for SARS-CoV-2 to enter the cell.


  • I worked with my partners Jenny and Carolyn to research and create presentation.
  • I used the Week 14 Protocol for this assignment.
  • Except for what is noted above, this individual journal entry was completed by me and not copied from another source.

Non (talk) 23:14, 29 April 2020 (PDT)