Imperial College/Courses/Spring2008/Synthetic Biology/SB Lecture 4

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Synthetic Biology Lectures

• Topic 1
• Topic 2
• Topic 3
• Topic 4
• Topic 5

Biological part characterisation and quality control

Instructor: Vincent Rouilly

Date: 21th February 2008

Venue: Room 1, Whiteleys Suite, RSC1 building.

Objectives:

importance of quality control and characterisation in Engineering (examples from electronic / mechanical systems) Checking DNA integrity BioBrick characterisation (promoter, RBS, Protein ...) + concept of a Registry of standard biological parts. Review on BioBrick F2620 chacacterisation Chassis characterisation (Imperial iGEM 2007)

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Presentation Slides:

• Course Slides (PDF, 2M)

Resources / References :

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• F2620 Characterisation (registry)
• Measurement Kit (Endy Lab)
  • Video from Jason Kelly introducing a standardized measurement kit for characterizing promoters and RBSs

• Screening Plasmid (Endy Lab)
• Cell-Free Chassis (Imperial iGEM 2007)