Imperial College/Courses/Spring2008/Synthetic Biology/Part Measurement and Characterisation/Resources
<html> <body> <!-- Start of StatCounter Code --> <script type="text/javascript"> var sc_project=3315864; var sc_invisible=0; var sc_partition=36; var sc_security="8bb2efcd"; </script>
<script type="text/javascript" src="http://www.statcounter.com/counter/counter_xhtml.js"></script><noscript><div class="statcounter"><a class="statcounter" href="http://www.statcounter.com/"><img class="statcounter" src="http://c37.statcounter.com/3315864/0/8bb2efcd/0/" alt="blog stats" /></a></div></noscript> <!-- End of StatCounter Code -->
</body> </html>
Wet Lab: Part Measurements and Characterisation
Calibrated Measurements and Calibration Curves:
- OD600 versus viable cell counting (serial plate dilution)
- Fluorescence measured versus purified protein concentration.
- Fluoresence measured 'In Vivo' versus Fluorescence measured 'In Vitro' (happy cells and lysed cells)
- Detailed protocols to be given: plate reader data analysis, [[
- Collect data, and document their work in their OWW lab notebook.
- Useful Protocols:
Promoter Characterisation using a standardised measurement kit
- Each group is given X promoters to sort out by strength.
- Detailed protocol to be given.
- Collect data, and document their work in their OWW lab notebook.
- BioBrick candidates:
- <bbpart>I20259</bbpart>: Constitutive_Promoter_1 (from combinatorial library) --GFPmut3--XXX
- <bbpart>I20260</bbpart>: Constitutive_Promoter_2 (from combinatorial library) --GFPmut3--XXX
- <bbpart>I20261</bbpart>: Constitutive_Promoter_3 (from combinatorial library) --GFPmut3--XXX
- <bbpart>I7101</bbpart>: pTetR--GFPmut3--XXX
- <bbpart>I7107</bbpart>: pLacI--GFPmut3--XXX
- <bbpart>I7109</bbpart> pCI--GFPmut3--XXX
- Useful protocols:
Inducible Promoter Characterisation
- Using the previous protocols, students are expected to define their own standard protocol.
- Collect data and document their work in their OWW lab notebook.
- Model expression system, Analyze Data.
- BioBrick candidates:
- <bbpart>I0500</bbpart>: pBAD promoter (only)
- <bbpart>I13520</bbpart>: pBAD--mRFP--XXX
- <bbpart>I6058</bbpart>: pBAD--EYFP--XXX
- <bbpart>I13540</bbpart>: pBAD--GFPmut3--XXX
- Useful Protocols:
Inverter Characterisation
- From the previous protocols students have to define their own standard protocol.
- Collect data and document their work in their OWW lab notebook.
- Model expression system, and Analyze Data.
- BioBrick candidates:
- <bbpart>J45996</bbpart>: Exponential-Phase-Sensitive GFP Generator
- <bbpart>J45995</bbpart>: Stationary-Phase-Sensitive GFP Generator
- <bbpart>J5517</bbpart>: pBAD--LacIts--XXX--pLacI--GFP--XXX
- <bbpart>E0611</bbpart>: pBAD--tetR-Inverter--EYFP--XXX
- <bbpart>E0610</bbpart>: pBAD--LacI-Inverter--EYFP--XXX
- <bbpart>E0612</bbpart>: pBAD--cI-Inverter--EYFP--XXX
- <bbpart>pSB1A10</bbpart>: Screening plasmid (GFP-inputs/RFP-outputs)+ TetR-Inverter
- Useful Protocols:
Resources on OpenWetWare:
Resources on the web