IGEM:Harvard/2006/Container Design 5/EM

• Project Overview
• Designs
• Notebook
• Protocols
• Presentations
• Literature

• Design 1
• Design 2
• Design 3
• Design 4
• Design 5
• Design 6

• Design 5 Overview
• Schematics
• Scaffold
• Code
• Latches
• Final Oligo List
• EM images

Tuesday, 2006 August 1

• Barrels folded using p7308

• 

  c5.0 barrel (3.3 nM), 6hb (3.3 nM), 0.2% uranyl formate

• 

  CG version of one barrel displayed to left.

• 

  .. and with scales (rough calculations; tilting of barrel reduces accuracy)

• 

  c5.0 barrel (3.3 nM), 6hb (3.3 nM), 0.2% uranyl formate

• 

  c5.0 barrel (3.3 nM), 6hb (3.3 nM), 0.2% uranyl formate

• 

  CG version of one barrel displayed to left.

• 

  .. and with scales (rough calculations; tilting of barrel reduces accuracy)

• 

  c5.0 barrel (3.3 nM), 6hb (3.3 nM), 0.2% uranyl formate

• 

  c5.0 barrel (3.3 nM), 6hb (3.3 nM), 0.2% uranyl formate

• 

  c5.0 barrel (3.3 nM), 6hb (3.3 nM), 0.2% uranyl formate

• Lid2 folded using p7704

• 

  c5.0 lid2 (2 nM), 6hb (0.4 nM), 0.2% uranyl formate

• 

  CG vision of possible lid to left

• 

  c5.0 lid2 (2 nM), 6hb (0.4 nM), 0.2% uranyl formate

• 

  c5.0 lid2 (2 nM), 6hb (0.4 nM), 0.2% uranyl formate

• 

  c5.0 lid2 (2 nM), 6hb (0.4 nM), 0.2% uranyl formate

Friday, 2006 August 18

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

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  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate

• 

  c5.0 barrel (10 nM), 0.7% uranyl formate