BioMicroCenter:Tecan Freedom Evo

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HOME -- SEQUENCING -- LIBRARY PREP -- HIGH-THROUGHPUT -- COMPUTING -- OTHER TECHNOLOGY

** All users must be trained before being allowed to use the Tecans **
The BioMicro Center currently hosts three automated liquid handlers: two Tecan Freedom EVO 150s and one TTP Labtech Mosquito HV. One EVO is optimized for cherry picking applications while the other is optimized for screening. The Tecans and Mosquito in the BioMicro Center are available to MIT researchers for use in their research. The Tecans may be used for BL1 work but are not set up for doing work on tissue culture or other samples requiring sterile environments. For users requiring higher throughput or BL2/BL2+ a new High-Throughput core has been developed by the Koch Institute.

Usage of the automated liquid handles is offered as a walk up service or as an assisted service. Due to the steep learning curves on the robots, we strongly encourage users to allow BMC staff to assist them in automating protocols and programming the instruments and then simply execute the programs as a walk up. Machine downtime is extremely limited and there is minimal time for practice runs.

JOHNNY 5

Instrument TECAN EVO 150
TYPE WALKUP - MIT only
Approval required
UNIT Per Hour + Consumables
ARMS
  • LiHa span4 (2uL-200uL)
  • MCA96 (50uL/200uL tips)
CARRIERS
  • 1 fixed tip washer.
  • 3 15ml tube carriers.
  • 3 temperature controlled SBS carriers
  • 24 non-temperature controlled SBS carriers
  • 4 racks for 1.5ml tubes (24 each)
  • UV light for decontamination
SIGNUP ilabs
NEW USERS New users should request training by emailing biomicro@mit.edu
UPGRADES BY Department of Biology

"Johnny5" or "J5" is the BioMicro Center robot optimized for cherry picking. It is a highly-versatile device equipped with a four-channel liquid handling arm (LiHa) and a 96-channel pipette arm (MCA96). The instrument supports a wide variety of fluid transfer operations for all sizes of microplates as well as 150mm dishes and can perform operations including reagent addition, continuous reagent dispensing, sample transfer, plate replication, plate-to-plate transfer, serial dilutions, and within-well mixing. The EVO can accurately handle volumes between 1 and 500 µl and has the ability to perform virtually any routine liquid transfer, replacing manual pipetting.

J5 is used by the BioMicro Center for plate normalization and SYBRgreen setup as part of high-throughput library production. Users should plan experiments in advance to coordinate usage with BMC staff.

BMC Johnny5 robot.jpg


BMC Johnny5.jpg

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B9

Instrument TECAN EVO 150
TYPE WALKUP - MIT only
Approval required
UNIT Per Hour + Consumables
ARMS
  • MCA96 (50uL/200uL tips)
  • RoMA (manipulator arm)
CARRIERS
  • 1 rack for 2 stacks of nested tips with chute
  • 3 temperature controlled SBS format positions on one carrier.
  • 16 SBS format positions on 4 carrier
  • Vortexer (Te-Shake)
  • 4 on deck racks for short plates
  • Shaking Incubator
  • 4 Stackers (TeStack)
  • Alpaqua 96w Magnets
SIGNUP ilabs
NEW USERS New users should request training by emailing biomicro@mit.edu
DONATED BY Dr. WENDY GILBERT (now at Yale)

"B9" is the BioMicro Center EVO150 optimized for high throughput screening. B9 has a 96 tip head for rapid pipetting and a robotic arm for moving plates around the deck. The robot has 4 plate stackers which can be used to add or remove plates during a run. B9 was donated to the BioMicro Center by Dr. Wendy Gilbert through a shared instrumentation grant from the NIH.

B9 is used by the BioMicro Center for qPCR setup, Illumina Library preparation, and SPRI bead cleans.

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BMC B9.png

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TTP LABTECH MOSQUITO HV

Instrument MOSQUITO HV
TYPE WALKUP
Approval required
UNIT Per Hour + Consumables
ARMS 8 tip disposable (0.2 - 5uL)
CARRIERS 5 SBS positions
SIGNUP contact biomicro@mit.edu
DONATED BY TTP LABTECH (on loan)

The TTP Labtech Mosquito HV is a critical instrument in the BioMicro Center's goal of automated high-throughput library production. The accurate low volume pipetting offered by this liquid handler has allowed for miniaturization of a number of protocols. While the instrument does not provide a 'walk away' level of automation, programming the Mosquito is significantly easier than the Tecans and prototyping methods is much more rapid.

The Mosquito is used in all HTL protocols in the BioMicro Center.

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