BISC209: Stock cultures

Stock Cultures

Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)

Lab 4:
Gram pos: PEA growth
Staph epi.

Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.

Organism	Colonial Appearance
Escherichia coli	purple with black center/ green metallic sheen
Klebsiella pneumoniae	dark centered colonies/ green metallic sheen
Enterobacter aeorogenes	pink colonies/ no metallic sheen
Proteus mirabilis	colorless colonies
Salmonella typhimurium	colorless colonies

Lab 5

catalase test

• positive control:
  Bacillus or Staph. epi
  
  Negative control:
  Enterococcus faecalis
  

oxidase test

• Escherichia coli

Antibiotic plates control orgs (2 Gram pos 1 Gram neg):

• Eschericia coli (Gram negative), Staphylococcus epidermidis (Gram positive) and Micrococcus luteus
  
  Starch plate
  • pos. controls Bacillus subtilis
    neg control: Staph. sp.?
    

  Lab 6
  

  MRVP TEST
  • MR pos control: Escherichia coli
    VP pos control: Enterobacter aerogenes

  Links to Labs

  Lab 1
  Lab 2
  Lab 3
  Lab 4
  Lab 5
  Lab 6
  Lab 7
  Lab 8
  Lab 9
  Lab 10
  Lab11
  Lab 12