BISC209: Stock cultures

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Wellesley College-BISC 209 Microbiology -Spring 2010

Stock Cultures

Lab 1:
Staphylococcus epidermidis
Serratia marcescens maintained at both 27 (red) and 37 (white)

Lab 4:
Gram pos: PEA growth
Staph epi.

Gram neg EMB growth
any of these:
Table 2. Colonial appearance on EMB Agar after 18-24 hours at 35°C. A differential medium can be used to differentiate Gram negative enteric organisms based on the colony color.

Organism Colonial Appearance
Escherichia coli purple with black center/ green metallic sheen
Klebsiella pneumoniae dark centered colonies/ green metallic sheen
Enterobacter aeorogenes pink colonies/ no metallic sheen
Proteus mirabilis colorless colonies
Salmonella typhimurium colorless colonies

Lab 5

catalase test
  • positive control:
    Bacillus or Staph. epi

    Negative control:
    Enterococcus faecalis
oxidase test
  • Escherichia coli
Antibiotic plates control orgs (2 Gram pos 1 Gram neg):