NanoBio:Protocols: Difference between revisions
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[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | ||
==Lab | ==Lab Manual== | ||
*[[NanoBio: Notebook| Keeping a Laboratory Notebook]] | *[[NanoBio: Notebook| Keeping a Laboratory Notebook]] | ||
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*[[NanoBio: Commonly Used Primers | Commonly Used Primers]] | *[[NanoBio: Commonly Used Primers | Commonly Used Primers]] | ||
*[[NanoBio: PCR | PCR]] | *[[NanoBio: PCR | PCR]] | ||
*[[NanoBio: Golden Gate Cloning | Golden Gate Cloning]] | |||
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | *[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]] | ||
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | *[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]] | ||
| Line 24: | Line 25: | ||
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]] | *[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]] | ||
*[[NanoBio: Plasmid Verification | Plasmid Verification]] | *[[NanoBio: Plasmid Verification | Plasmid Verification]] | ||
*[[NanoBio: Archiving Your Plasmid in the Strain Collection | Archiving Your Plasmid in the Strain Collection]] | |||
*[[NanoBio: AgaroseGels | Agarose Gels]] | *[[NanoBio: AgaroseGels | Agarose Gels]] | ||
| Line 67: | Line 69: | ||
* [[NanoBio: BG-11 plates| BG-11 plates]] | * [[NanoBio: BG-11 plates| BG-11 plates]] | ||
* [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]] | * [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]] | ||
* [[NanoBio: Stock Solutions | Stock Solutions]] | |||
==Safety & Waste Disposal== | ==Safety & Waste Disposal== | ||
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]] | *[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]] | ||
Latest revision as of 16:12, 1 May 2013
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Lab Manual
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
- 3 Antibiotic Assembly
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Golden Gate Cloning
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Bacterial Transformation of Chemically Competent Cells
- Prep for Electrocompetent cells
- Bacterial Transformation using Electroporation
- Plasmid Verification
- Archiving Your Plasmid in the Strain Collection
- Agarose Gels
Protein Expression & Purification
- Bacterial Hosts for Overexpression
- Test Induction & Purification
- Protein Purification
- Isolation of Periplasmic Fraction
- Protein Gels
- Cell Surface Binding Assay
Gene deletion with λ-red
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
Recipes
- LB media & plates
- Antibiotics
- TSB media
- BG-11 media
- BG-11 plates
- Supplemented M9 Media
- Stock Solutions
