20.109: Spring 2007 schedule
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| MODULE | DAY | DATE | LECTURER | TA | LAB | note |
| Tue Feb 6 | NK | Orientation | ||||
| 1 | 1 | Thur Feb 8 | DE | SC | Start-up genome engineering | PDF of class discussion slides |
| 1 | 2 | Tue Feb 13 | DE | SC | Agarose gel electrophoresis | PDF of class notes |
| 1 | 3 | Thur Feb 15 | DE | SC | DNA ligation and bacterial transformation | |
| President's Day Feb 19 | No lab Feb 20 or Feb 21 | |||||
|---|---|---|---|---|---|---|
| 1 | 4 | Thur Feb 22 | DE | SC | Examine candidate clones | guest lecture from Harlan Breindel |
| 1 | 5 | Tue Feb 27 | DE | SC | Western analysis | PDF of class discussion slides |
| 1 | 6 | Thur Mar 1 | DE | SC | Probe western | first draft of your writing assignment due before lab, PDF of class slides |
| 2 | 1 | Tue Mar 6 | AJ | YSN | Start-up signal measurement | |
| 2 | 2 | Thur Mar 8 | AJ | YSN | Measuring calcium in vitro | |
| 2 | 3 | Tue Mar 13 | AJ | YSN | Lipofection | |
| 2 | 4 | Thur Mar 15 | AJ | YSN | Measuring calcium in vivo | |
| 2 | 5 | Tue Mar 20 | AJ | YSN | Calcium signaling in vivo | |
| 2 | 6 | Thur Mar 22 | AJ | YSN | Student presentations | |
| Spring Break Mar 26-30 | No lab all week | |||||
| 3 | 1 | Tue Apr 3 | NK | AT | Start-up expression engineering | PDF of M3D1 lecture slides |
| 3 | 2 | Thur Apr 5 | NK | AT | Yeast transformation | PDF of M3D2 lecture slides |
| 3 | 3 | Tue Apr 10 | NK | AT | Colony PCR | guest lecture: Susan Ruff and her slides are here |
| 3 | 4 | Thur Apr 12 | NK | AT | Screen for phenotypes, isolate RNA | PDF of M3D4 lecture slides |
| Patriot's Day April 16-17 | No lab Apr 17 or Apr 18 | |||||
| 3 | 5 | Thur Apr 19 | NK | AT | cDNA synthesis and microarray | PDF of M3D5 lecture slides |
| 3 | 6 | Tue Apr 24 | NK | AT | Microarray data analysis | guest lecture: Rebecca Fry and her slides are here |
| 4 | 1 | Thur Apr 26 | AB | CV | Screening library | |
| 4 | 2 | Tue May 1 | AB | CV | Optimizing panning | first draft of your writing assignment due Monday April 30th by 9AM |
| 4 | 3 | Thur May 3 | AB | CV | Rescreening gold binders | |
| 4 | 4 | Tue May 8 | AB | CV | PCR of gold binding candidates | Mod 3 lab reports returned for revision |
| 4 | 5 | Thur May 10 | AB | CV | Student presentations | |
| 4 | 6 | Tue May 15 | AB | CV | Analyze sequence data | Revised Mod 3 writing assignment due before lab no lecture today |
| Thur May 17 | Lecture only, no lab | |||||
