MODULE
|
DAY
|
DATE
|
LECTURER
|
TA
|
LAB
|
NOTES
|
|
|
Tue/Wed Feb 5/6
|
ANS
|
|
Orientation
|
* Introductory Lecture (PDF) * Intro Pre-Lab, T/R * Intro Pre-Lab, W/F
|
|
1
|
1
|
Thur/Fri Feb 7/8
|
JR
|
Ian
|
Context-setting and primer design
|
* M1D1 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
1
|
2
|
Tue/Wed Feb 12/13
|
JR
|
Ian
|
DNA extraction
|
* M1D2 Lecture * T/R Pre-Lab (green text added later) * W/F Pre-Lab T/R WAC talk W/F WAC talk
|
1
|
3
|
Thur/Fri Feb 14/15
|
JR
|
Ian
|
PCR and paper discussion
|
* M1D3 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
|
|
President's Day Feb 18
|
No lab Feb 19 or Feb 20
|
1
|
4
|
Thur/Fri Feb 21/22
|
JR
|
Ian
|
DNA cloning
|
* M1D4 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
1
|
5
|
Tue/Wed Feb 26/27
|
JR
|
Ian
|
DNA sequencing and primer analysis
|
* M1D5 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
1
|
6
|
Thur/Fri Feb 28/ Mar 1
|
JR
|
Ian
|
Journal club I
|
* M1D6 Lecture
|
1
|
7
|
Tue/Wed Mar 5/6
|
JR
|
Ian
|
Phylogenetic analysis
|
* M1D7 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
1
|
8
|
Thur/Fri Mar 7/8
|
NO LECTURE TODAY
|
|
Journal club II
|
|
|
2
|
1
|
Tue/Wed Mar 12/13
|
AJ
|
MM
|
Design mutant
|
* M2D1 Lecture * T/R Pre-Lab * W/F Pre-Lab Module 1 Draft Due
|
2
|
2
|
Thur/Fri Mar 14/15
|
AJ
|
MM
|
Site-directed mutagenesis
|
* M2D2 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
2
|
3
|
Tue/Wed Mar 19/20
|
AJ
|
MM
|
Bacterial amplification of DNA
|
* M2D3 Lecture *Module 1 Primer Summary Due * T/R Pre-Lab * W/F Pre-Lab
|
2
|
4
|
Thur/Fri Mar 21/22
|
AJ
|
MM
|
Prepare expression system
|
* M2D4 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
|
|
Spring Break Mar 25-29
|
No lab all week
|
2
|
5
|
Tue/Wed Apr 2/3
|
AJ
|
MM
|
Induce protein and evaluate DNA
|
* M2D5 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
2
|
6
|
Thur/Fri Apr 4/5
|
AJ
|
MM
|
Purify protein
|
* M2D6 Lecture * T/R Pre-Lab * W/F Pre-Lab Module 1 Revision Due
|
2
|
7
|
Tue/Wed Apr 9/10
|
AJ
|
MM
|
Characterize protein expression
|
* M2D7 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
2
|
8
|
Thur/Fri Apr 11/12
|
AJ
|
MM
|
Assess protein function
|
* M2D8 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
|
|
Patriot's Day April 15-16
|
No lab Apr 16 or Apr 17
|
|
3
|
1
|
Thur/Fri Apr 18/19
|
ANS
|
|
Design experiment
|
* M3D1 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
3
|
2
|
Tue/Wed Apr 23/24
|
ANS
|
|
Initiate cell culture
|
* M3D2 Lecture Module 2 Report Due MONDAY APRIL 22 by 5 pm for all.
|
3
|
3
|
Thur/Fri Apr 25/26
|
ANS
|
* M3D3 Lecture
|
Testing cell viability
|
|
3
|
4
|
Tue/Wed Apr 30/May 1
|
ANS
|
|
Preparing cells for analysis
|
* M3D4 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
3
|
5
|
Thur/Fri May 2/3
|
ANS
|
|
Initiating transcript and protein assays
|
* M3D5 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
3
|
6
|
Tue/Wed May 7/8
|
ANS
|
|
Transcript-level analysis
|
* M3D6 Lecture * T/R Pre-Lab * W/F Pre-Lab
|
3
|
7
|
Thur/Fri May 9/10
|
ANS
|
|
Protein-level and wrap-up analysis
|
Lecture: on Atissa's page * T/R Pre-Lab * W/F Pre-Lab Module 3 Summary Due
|
3
|
8
|
Tue/Wed May 14/15
|
ANS
|
|
Student presentations
|
|
|
|
|
Thur May 16
|
|
|
No lab session. Meet in 56-614 from 11am-1pm: feedback/discussion about class, then celebratory lunch etc.
|
|