User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/11

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Cellular Adhesion

  • Glycerol
    • Took 1.6ml of liquid culture and created 10% glycerol stocks
      • IgAbc #8
  • Miniprep
    • Extracted DNA from remaining liquid culture (~6ml)
    • Eluted in 50ul water
  • Liquid culture
    • Grew up cultures in 8ml Amp/Kan LB (didn't have mixed, so used 4ml of LB KAN and 4ml of LB Amp) inoculated from plates
      • IgAss + Fos #1
      • IgAss + Fos #2
      • IgAss + Fos #3
      • IgAss + JunB #1
      • IgAss + JunB #2
      • IgAss + JunB #3
    • Grew up cultures in 8ml Amp/Cl LB inoculated from plates
      • GCN4 #4
      • GCN4 #5
      • GCN4 #6
  • PCR
    • Template: 40ul PCR Supermix, .4ul VF2 and VR, 1ul IgAbc #8 miniprep
    • Same thermocycler protocol as 8.1.2007
Full IgA beta-core without muts, IgAbc #8, log-2 ladder
  • Gel
    • IgAbc #8 looks correct
    • Try sequencing it again