User contributions for Mbennie

25 January 2008

• 19:4119:41, 25 January 2008 diff hist +278 N User:Mbennie/Notebook/Sequencing/1.25.2008 New page: Template: 3ul Miniprep DNA, .3ul primer, 6.7ul water * 6His #1 with VF2 * 6His #2 with VF2 * FLAG #1 with VF2 * FLAG #2 with VF2 * Myc #1 with VF2 * Myc #2 with VF2 * HA #1 with VF2... current
• 19:3919:39, 25 January 2008 diff hist +59 User:Mbennie/Notebook/Sequencing →Sequencing Runs current
• 19:3819:38, 25 January 2008 diff hist +36 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/25 →[[Cellular Adhesion]] current
• 19:3719:37, 25 January 2008 diff hist +1,555 N User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/25 New page: ==Cellular Adhesion== * Gel ** Ran 1% gel for 25 minutes at 95V with samples (5ul sample with 2ul of loading dye) ** Looks good [[Image:Mbennie2008.1.25a.jpg|thumb|log-2 ladder, 6His 1...

24 January 2008

• 20:4420:44, 24 January 2008 diff hist +122 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/24 →[[Cellular Adhesion]] current
• 19:5019:50, 24 January 2008 diff hist +70 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/24 →[[Cellular Adhesion]]
• 19:3219:32, 24 January 2008 diff hist +271 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/24 →[[Cellular Adhesion]]
• 19:3119:31, 24 January 2008 diff hist +666 N User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/24 New page: ==Cellular Adhesion== * Colony PCR * Liquid Cultures ** Inoculated 8ml Amp/Cl LB with antibody tags and GCN4 from yesterday's plate colonies ** Grew up in 37C overnight * Gel ** Ran...

23 January 2008

• 22:4322:43, 23 January 2008 diff hist +687 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 No edit summary current
• 21:1021:10, 23 January 2008 diff hist +219 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 →[[Cellular Adhesion]]
• 20:5220:52, 23 January 2008 diff hist +30 User:Mbennie/Notebook/Oligos/NEW Mut2 Pst1b-R No edit summary current
• 20:5220:52, 23 January 2008 diff hist +30 User:Mbennie/Notebook/Oligos/NEW Mut2 Pst1b-F No edit summary current
• 20:5220:52, 23 January 2008 diff hist +30 User:Mbennie/Notebook/Oligos/NEW Mut Pst1a-R No edit summary current
• 20:5220:52, 23 January 2008 diff hist +30 User:Mbennie/Notebook/Oligos/NEW Mut Pst1a-F No edit summary current
• 20:5120:51, 23 January 2008 diff hist +190 N User:Mbennie/Notebook/Oligos/NEW Mut2 Pst1b-R New page: Sequence: GTTTCTTCgctcttcaagcagataacaagggttttacggtaaggt Binding site: 26 bp Predicted Tm: 54.3C Received: Late August 2007 (resuspended 1/23/2008) * Resuspended at 50uM with 459 ul of ...
• 20:5120:51, 23 January 2008 diff hist +189 N User:Mbennie/Notebook/Oligos/NEW Mut2 Pst1b-F New page: Sequence: GTTTCTTCgctcttcagctgcttactttgccaattatggcaa Binding site: 23 bp Predicted Tm: 55.2C Received: Late August 2007 (resuspended 1/23/2008) * Resuspended at 50uM with 421.2 ul of T...
• 20:5020:50, 23 January 2008 diff hist +187 N User:Mbennie/Notebook/Oligos/NEW Mut Pst1a-R New page: Sequence: GTTTCTTCgctcttcaggcagatgacaggcggctgtaacg Binding site: 21 bp Predicted Tm: 56.6C Received: Late August 2007 (resuspended 1/23/2008) * Resuspended at 50uM with 438.2 ul of TE
• 20:4920:49, 23 January 2008 diff hist 0 User:Mbennie/Notebook/Oligos →8/16/2007 current
• 20:4320:43, 23 January 2008 diff hist −4 User:Mbennie/Notebook/Oligos/NEW Mut Pst1a-F No edit summary
• 20:4320:43, 23 January 2008 diff hist +193 N User:Mbennie/Notebook/Oligos/NEW Mut Pst1a-F New page: Sequence: GTTTCTTCgctcttcagccgattacaagttgggtgacgacag Binding site: 23 bp Predicted Tm: 52.9C Received: Late August 2007 (resuspended 1/23/2008) * Resuspended at 50uM with 533.2 ul ...
• 20:4020:40, 23 January 2008 diff hist +287 User:Mbennie/Notebook/Oligos No edit summary
• 19:4819:48, 23 January 2008 diff hist +203 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 →[[Cellular Adhesion]]
• 19:4819:48, 23 January 2008 diff hist +1 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 No edit summary current
• 17:0517:05, 23 January 2008 diff hist +35 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 No edit summary
• 17:0417:04, 23 January 2008 diff hist +140 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 →[[Cellular Adhesion]]
• 17:0217:02, 23 January 2008 diff hist +426 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 No edit summary

22 January 2008

• 20:3220:32, 22 January 2008 diff hist +750 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/22 →[[Cellular Adhesion]] current
• 20:3220:32, 22 January 2008 diff hist −748 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 Removing all content from page
• 17:3317:33, 22 January 2008 diff hist +748 N User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/23 New page: * PCR ** Template: 40ul PCR Supermix, .8ul primers (.4ul primers for GCN4), .8ul DNA (only GCN4) *** 6His Tag: 6His_Tag-F and [[User:Mbennie/Not...
• 17:3317:33, 22 January 2008 diff hist −721 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/22 →[[Cellular Adhesion]]
• 16:5616:56, 22 January 2008 diff hist +2 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/22 →[[Cellular Adhesion]]
• 16:5516:55, 22 January 2008 diff hist +772 N User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/22 New page: ==Cellular Adhesion== * PCR ** Template: 40ul PCR Supermix, .8ul primers (.4 primers for GCN4), .8ul DNA (only GCN4) *** 6His Tag: [[User:Mbennie/Notebook/Oligos/6His_Tag-F|6His_Tag-F]...

21 January 2008

• 22:0422:04, 21 January 2008 diff hist +1 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 →[[Cellular Adhesion]]
• 22:0422:04, 21 January 2008 diff hist +103 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 →[[Cellular Adhesion]]
• 22:0222:02, 21 January 2008 diff hist +3 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 →[[Cellular Adhesion]]
• 22:0122:01, 21 January 2008 diff hist −2 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 →[[Cellular Adhesion]]
• 22:0122:01, 21 January 2008 diff hist +360 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 →[[Cellular Adhesion]]
• 21:4821:48, 21 January 2008 diff hist +436 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 →[[Cellular Adhesion]]
• 21:2921:29, 21 January 2008 diff hist +15 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 →[[Cellular Adhesion]]
• 20:4420:44, 21 January 2008 diff hist 0 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 No edit summary
• 20:4320:43, 21 January 2008 diff hist −11 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 No edit summary
• 20:4320:43, 21 January 2008 diff hist +2,169 User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 No edit summary
• 20:2020:20, 21 January 2008 diff hist +25 N User:Mbennie/Notebook/Lab Notebook/Notebook/2008/01/21 New page: ==Cellular Adhesion==

7 January 2008

• 17:1517:15, 7 January 2008 diff hist 0 User:Mbennie/Notebook/Lab Notebook No edit summary current
• 17:1417:14, 7 January 2008 diff hist 0 User:Mbennie/Notebook/Lab Notebook No edit summary

19 August 2007

• 22:5522:55, 19 August 2007 diff hist +466 User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/19 →[[Cellular Adhesion]] current
• 22:4922:49, 19 August 2007 diff hist +152 User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/19 →[[Cellular Adhesion]]
• 17:0017:00, 19 August 2007 diff hist +119 User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/19 →[[Cellular Adhesion]]
• 16:5916:59, 19 August 2007 diff hist +805 N User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/19 New page: ==Cellular Adhesion== * Liquid Culture ** Inoculated 8ml Amp/Cl LB with antibody tags and GCN4 from plate colonies ** Inoculated 8ml Amp/Kan LB with autotransporter from plate colonies...
• 14:4514:45, 19 August 2007 diff hist +35 User:Mbennie/Notebook/Lab Notebook/Notebook/2007/08/18 →[[Cellular Adhesion]] current