User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/28

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Objective

Cassidy and Sarah got strange results for their fluorescence measurements yesterday (myoglobin, AuNPs, pH4). So I'm going to re run their samples.

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock solution for class

  1. AgNO3
    1. 28.13 mg AgNO3
    2. to 10.0 mL in water
    3. 169.87 g/mol
    4. 16.6 mM

Myoglobin Sample Prep

  1. 250 μM AuCl3, 12.5 μM myoglobin, pH 4
    1. 176 μL 4.27 mM AuCl3
    2. 234 μL 160 μM myoglobin
    3. 300 μL 1mM HCl
    4. 2290 μL water


UV-Vis of sample before and after measurement

20160928 mrh MyoglobinAuNPUVVis.png

Fluorescence data

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

20160928 mrh MbAuNP IntegratedI.png

20160928 mrh MbAuNP EmissionMax.png

20160928 mrh MbAuNP selectedscans1.png

20160928 mrh MbAuNP selectedscans2.png

20160928 mrh MbAuNP selectedscans3.png

Notes

Anneliese Faustino

Hannah Harvey

Sara Mattson

Patrick Hampson

Sarah Burkhard