User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/29

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Objective

Run fluorescence scans for

  1. BSA, AuNP, pH 6, 80C
  2. Lysozyme, pH 5, 80C

BSA AuNP Fluorescence

3 mL Sample

  1. 0.25 mM AuCl3
    1. Stock concentration 4.27 mM
    2. Volume Added: 176 μL
  2. 3.125 μM BSA
    1. Stock Concentration 52.6 μM
    2. Volume Added: 178 μL
  3. pH 6
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 3 μL
  4. Volume water added: 2643 μL

UV-Vis of sample

20160929 mrh BSA AuNP UVVis.png

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

20190929 mrh BSAAuNP IntegratedI.png

20190929 mrh BSAAuNP EmissionMax.png

20190929 mrh BSAAuNP scans1.png

20190929 mrh BSAAuNP scans2.png

20190929 mrh BSAAuNP scans3.png


Lysozyme Fluorescence

3 mL Sample

  1. 0.5 μM Lysozyme
    1. Stock Concentration 210 μM
    2. Volume Added: 7.1 μL
  2. pH 5
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 30 μL
  3. Volume Water Added: 2963 μL

UV-Vis of sample

20160929 mrh Lysozyme pH5 UVVis.png

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

20160929 mrh LysozymepH5 IntegratedI.png

20160929 mrh LysozymepH5 EmissionMax.png

20160929 mrh LysozymepH5 SelectedScans.png

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


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