User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/29

From OpenWetWare
Jump to navigationJump to search
Error creating thumbnail: Unable to save thumbnail to destination Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

Run fluorescence scans for

  1. BSA, AuNP, pH 6, 80C
  2. Lysozyme, pH 5, 80C

BSA AuNP Fluorescence

3 mL Sample

  1. 0.25 mM AuCl3
    1. Stock concentration 4.27 mM
    2. Volume Added: 176 μL
  2. 3.125 μM BSA
    1. Stock Concentration 52.6 μM
    2. Volume Added: 178 μL
  3. pH 6
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 3 μL
  4. Volume water added: 2643 μL

UV-Vis of sample

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements


Lysozyme Fluorescence

3 mL Sample

  1. 0.5 μM Lysozyme
    1. Stock Concentration 210 μM
    2. Volume Added: 7.1 μL
  2. pH 5
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 30 μL
  3. Volume Water Added: 2963 μL

UV-Vis of sample

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.