User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/28

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Objective

  1. Run Ocean Optics for Lysozyme protein with a pH of 6.

Description

  1. Make solution for cuvette. Concentrations can be found in the Data section below.
  2. Run Ocean Optics data every two minutes for 3 hours.

Data

Stock solutions

  1. Lysozyme
    1. concentration = 210 μM
  2. AuCl3
    1. concentration = 4.27 mM

Instructions for making sample for ocean optics

  1. pH 6
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 176 μL AuCl3
      2. 3 μL 1M HCl
      3. 179 μL Lysozyme
      4. 2642 μL Water
092816 Wave pH 6.png

Figure 1 Values taken with lysozyme at a pH of 6

092816 Abs pH 6.png

Figure 2 Values taken with lysozyme at a pH of 6

092816 Abs Wave pH 6.png

Figure 3 Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 6. Values shown every 30 minutes. In addition, values shown at t=0-4 min t=32-34 min due to the large jump observed in Figure 1