User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/28

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Objective Run Ocean Optics for Lysozyme protein with a pH of 6. Description Make solution for cuvette. Concentrations can be found in the Data section below. Run Ocean Optics data every two minutes for 3 hours. Data Stock solutions Lysozyme concentration = 210 μM AuCl3 concentration = 4.27 mM Instructions for making sample for ocean optics pH 6 Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total 176 μL AuCl3 3 μL 1M HCl 179 μL Lysozyme 2642 μL Water Figure 1 Values taken with lysozyme at a pH of 6 Figure 2 Values taken with lysozyme at a pH of 6 Figure 3 Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 6. Values shown every 30 minutes. In addition, values shown at t=0-4 min t=32-34 min due to the large jump observed in Figure 1