User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/28

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Fluorescence Round 3 pH 6 automated set up, scanning every 180 seconds (from previously every 5 minutes) Concentrations and Volumes BSA Fluorescence Solution pH 6 [Au]=0.25 mM [BSA]=3.125 uM Vf=3000 uL Stock Concentrations 160920 [BSA]= 52.6 uM [Au]= 4.27 mM [HCl]= 1mM Volumes for making the solution: 3 uL HCl 294.6 uL BSA 292.7 uL Au 2409.5 uL H20 The concentrations and volumes were not accurately scaled to 3 mL. In the future, we will use automated calculations with a spreadsheet that we uploaded on Dropbox: "Stock Solution Calculation Template" volume ratio reductant : metal source (see above) = [BSA] : [Au] = 294.6/292.7 = 1.006 Hartings ratio: 178/176 = 1.01 Data Analysis Performed by Dr Matt Hartings: 3 mL Sample 0.25 mM AuCl3 Stock concentration 4.27 mM Volume Added: 176 μL 3.125 μM BSA Stock Concentration 52.6 μM Volume Added: 178 μL pH 6 Stock Concentration 1 mM HCl Volume Added: 3 μL Volume water added: 2643 μL UV-Vis of sample Fluorescence scans Acquisition information Excitation: 290 nm, 10 nm slit width Emission: 310-540 nm, 10 nm slit width 100 nm/minute 61 measurements, 180 second delay between measurements Notes Find data on Fluorescence computer : C:\WINLAB\DATA save each file individually as ASKII and .txt