User:Sarah Burkhard/Notebook/471 Nano Notebook/2016/09/28

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Fluorescence Round 3

pH 6

automated set up, scanning every 180 seconds (from previously every 5 minutes)

Concentrations and Volumes

BSA Fluorescence Solution

  1. pH 6
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 52.6 uM
  2. [Au]= 4.27 mM
  3. [HCl]= 1mM

Volumes for making the solution:

  1. 3 uL HCl
  2. 294.6 uL BSA
  3. 292.7 uL Au
  4. 2409.5 uL H20

The concentrations and volumes were not accurately scaled to 3 mL. In the future, we will use automated calculations with a spreadsheet that we uploaded on Dropbox: "Stock Solution Calculation Template"

volume ratio reductant : metal source (see above) = [BSA] : [Au] = 294.6/292.7 = 1.006

Hartings ratio: 178/176 = 1.01

Data Analysis

Performed by Dr Matt Hartings:


3 mL Sample

  1. 0.25 mM AuCl3
    1. Stock concentration 4.27 mM
    2. Volume Added: 176 μL
  2. 3.125 μM BSA
    1. Stock Concentration 52.6 μM
    2. Volume Added: 178 μL
  3. pH 6
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 3 μL
  4. Volume water added: 2643 μL

UV-Vis of sample

20160929 mrh BSA AuNP UVVis.png

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

20190929 mrh BSAAuNP IntegratedI.png

20190929 mrh BSAAuNP EmissionMax.png

20190929 mrh BSAAuNP scans1.png

20190929 mrh BSAAuNP scans2.png

20190929 mrh BSAAuNP scans3.png

Notes

Find data on Fluorescence computer : C:\WINLAB\DATA

save each file individually as ASKII and .txt