User:Andy Maloney/Temperature effects on kinesin velocity
Motivation
The purpose of this page is to look into the initial rise in speed I have been observing for all my experiments. It has been brought to my attention that this could be due to temperature affects. In order to rule out or confirm this idea, I've created this page that will discuss my findings.
Experiment
Notebook
Below you will find a list of my notebook entries related to investigating kinesin speed effects due to temperature changes.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/24/Objective temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Mapping out what I should do about stabilizing the temperature of the objective. Also, this page describes my initial study on temperature affects.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/More on kinesin speed effects due to temperature
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: his is a follow up to the initial study of temperature issues.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/Speeding up kinesin-driven microtubule gliding in vitro by variation of cofactor composition and physicochemical parameters
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/25/LED illumination for video-enhanced DIC imaging of single microtubules
- Categories: AM Temp effects on kinesin velocity, Kinesin and microtubule papers, Kinesin and microtubules
- Description: Paper review. LED illumination for video-enhanced DIC imaging of single microtubules
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/05/27/Casein data revisited
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules, Surface passivation
- Description: Reanalyzing the casein data I took to incorporate the times I took the data at. This shows a temperature affect going on.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/07/LED for microscope temperature control
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: This is my first look at how the Thorlabs LED controller works. The goal is to replace the existing LED with another LED so that I can get a broad spectrum of colors in the microscope.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/17/Temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Objective temperature stabilization build, slide warmer, and LED discussions.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/23/Temperature data
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Posting data with temperature stabilization as well as looking more at the LED from Bridgelux. Also, an experiment where I look at only one spot in the sample.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/06/29/Whole casein with temperature stabilization
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: I now have repeated experiments where I have stabilized the temperature of the objective and use whole casein as the passivation.
- Andy Maloney/Notebook/Lab Notebook of Andy Maloney/2010/07/19
- Categories: AM Surface passivation paper, AM Temp effects on kinesin velocity, Kinesin and microtubules
- Description: Beginning to notice that my casein solutions are no longer working. All I changed was the temperature stabilization.
References
All references that I read that I believe are important to the project will be listed here. I would like to state that the notes attached to a reference are my notes and thoughts. They may not be applicable to you or, I may have overlooked something important in the article that you may think is crucial for your research. With that said, please do read the reference before reading my notes and please, do not think my notes are in any way correct without referencing the article I am reviewing.
