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BISC220/S12: Mod 2 Media Recipes: Difference between revisions
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→Yeast Series Media Recipes
(New page: {{Template:BISC220/S12}} <div style="padding: 10px; width: 720px; border: 5px solid #ffff33;"> Background Information<br> [[BISC220/S12: Mod 2 Lab 6 ...) |
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| 0.67% Yeast Nitrogen Base <br> 2% Glucose <br> 0.078% -Ura -His amino acid drop out mix <br> 0.003M Histidinol <br> 2% Agar (only in solid media) | | 0.67% Yeast Nitrogen Base <br> 2% Glucose <br> 0.078% -Ura -His amino acid drop out mix <br> 0.003M Histidinol <br> 2% Agar (only in solid media) | ||
|- | |- | ||
! Yeast 5X Sample Buffer | ! Yeast 5X Protein Sample Buffer | ||
| 10% SDS <br> 25% Glycerol <br> 250mM Tris (pH = 6.8) <br> 500mM DTT <br> 0.5% BPB | | 10% SDS <br> 25% Glycerol <br> 250mM Tris (pH = 6.8) <br> 500mM DTT <br> 0.5% BPB | ||
|- | |- | ||
! Acrylamide Gel Tank Buffer (Running) | ! Acrylamide Gel Tank Buffer (Gel Running) | ||
| 0.92 M Glycine <br> 0.05M Trisma base <br> 0.1% SDS | | 0.92 M Glycine <br> 0.05M Trisma base <br> 0.1% SDS | ||
|- | |- | ||
! Western Blotting Buffer | ! Western Blotting Buffer (Nitrocellulose Transfer) | ||
| 25mM Trisma Base <br> 192mM Glycine <br> 20% v/v Methanol | | 25mM Trisma Base <br> 192mM Glycine <br> 20% v/v Methanol | ||
|- | |- | ||
