BISC220/S12:Series 1
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Lab 1: Induction of β-galactosidase and Molecular Modeling
Lab 2: Affinity Chromatography and Specific Activity Determination
Lab 3: Enzyme Kinetics
The Michaelis-Menten Model
Lab 4: Polyacrylamide Gel Electrophoresis
Media Recipes
Science Writing and Figure Design
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 1 | 1/31 - 2/3 | 1) Induction of β-galactosidase 2) Molecular Modeling and Sequence Analysis |
Figure creation and brief analysis of the β-galactosidase molecule |
| 2 | 2/7 - 2/10 | 1) Affinity Chromatography 2) Determination of the Specific Activity of β-galactosidase |
Calculations based on the results of today's lab |
| 3 | 2/14- 2/17 | Enzyme Kinetics | Calculations based on the results of today's lab |
| 4 | 2/21 - 2/24 | Polyacrylamide gel electrophoresis | 1) Calculations based on the results of today's lab 2) Write β-galactosidase paper |
