BISC220/S12:Series 3
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Lab 8: Cell Culture
Lab 9: Apoptosis - DNA
Lab 10: Apoptosis - Protein 1
Lab 11: Apoptosis - Protein 2
Lab 12: Imaging Presentations
Media Recipes
Schedule of Experiments
| Lab # | Dates | Activity | Outside lab time |
|---|---|---|---|
| 8 | 3/27 - 3/30 | Introduction to Cell Culture | Cell passage and maintenance Work on Paper 2 |
| 9 | 4/3 - 4/6 | 1) Isolation of HL-60 genomic DNA 2) Agarose Gel Electrophoresis 3) Paper 2 due |
Find paper for Presentations |
| 10 | 4/10 - 4/13 | 1) Preparation of Protein Extracts 2) SDS-PAGE Gel 3) Examine fixed HL-60 cells |
Work on Presentations and Final Paper |
| 11 | 4/17 - 4/27 | PARP-1 Western Blot | Work on Presentations and Final Paper |
| 12 | 5/1 - 5/4 | Imaging Presentations | Final Paper due for everyone - Friday, May 4, 2012 |
