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BISC209: Enrichment: Difference between revisions
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→Enrichment media and Isolation protocol (Starting with plant leaves)
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Use Methanol Oxidizer medium'''</font color="purple"><BR> | Use Methanol Oxidizer medium'''</font color="purple"><BR> | ||
The organisms in this group are able to use C<sub>1</sub> carbon compounds for their energy and carbon requirements. There are specific and specialized metabolic pathways associated with this ability. There are a few distinct subgroups of methylotrophs within the ''proteobacteria'' and ''firmicutes''. We are interested in those subgroups that are capable of using methanol. We will attempt to select for these. Methanol users play an important role in | The organisms in this group are able to use C<sub>1</sub> carbon compounds for their energy and carbon requirements. There are specific and specialized metabolic pathways associated with this ability. There are a few distinct subgroups of methylotrophs within the ''proteobacteria'' and ''firmicutes''. We are interested in those subgroups that are capable of using methanol. We will attempt to select for these. Methanol users play an important role in carbon cycling. One genus, ''Methylobacterium'', is a common epiphyte on plants. In culture it produces pink-pigmented colonies. We will attempt to isolate this genus directly from leaves. | ||
<UL><LI> | <UL><LI> | ||
'''Enrichment:'''<BR> | '''Enrichment:'''<BR> | ||
