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BISC209: Enrichment: Difference between revisions
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→Enrichment media and Isolation protocols (Starting with SOIL EXTRACT)
Tucker Crum (talk | contribs) |
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Make a wet mount from the colored or spreading colones or prepare a hanging drop slide for viewing with your brightfield microscope. Look for rod shaped bacteria with tapering or rounded ends. <LI> | Make a wet mount from the colored or spreading colones or prepare a hanging drop slide for viewing with your brightfield microscope. Look for rod shaped bacteria with tapering or rounded ends. <LI> | ||
'''Isolation:''' <BR> | '''Isolation:''' <BR> | ||
Purify the strains you suspect are '' | Purify the strains you suspect are ''Gliders'' by transfering material from the edge of a pigmented, swarming colony to 2 ml of cytophagia medium in a small tube and vortex to separate the cells.<LI> | ||
Then follow [[BISC209: Streaking for Isolation | Streaking for Isolation ]] on | Then follow [[BISC209: Streaking for Isolation | Streaking for Isolation ]] on Cytophagia Agar plates<LI> | ||
continue sub-culturing until you have pure isolates.<LI> | continue sub-culturing until you have pure isolates.<LI> | ||
'''Identification:'''<BR> | '''Identification:'''<BR> | ||
