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BISC209: Enrichment: Difference between revisions
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→Enrichment media and Isolation protocols (Starting from a Colony from your Standard Plate Count, or a colony from the initial inoculation on general purpose media: NA, or diluteNA )
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<UL><LI> | <UL><LI> | ||
'''Enrichment:'''<BR> | '''Enrichment:'''<BR> | ||
Follow the instructions for [[BISC209: Serial Dilution | Serial Dilution ]] of the Soil Extract | Follow the instructions for [[BISC209: Serial Dilution | Serial Dilution ]] of the Soil Extract onto nutrient agar. <LI> | ||
After incubating the plates for 24, 36, etc. hours examine the 10<sup>-3</sup> and/or 10<sup>-4</sup> plates until you see likely candidates of ''Bacillus'' colonies based on the colony description and Gram or Spore stains.<LI> | After incubating the plates for 24, 36, etc. hours examine the 10<sup>-3</sup> and/or 10<sup>-4</sup> plates until you see likely candidates of ''Bacillus'' colonies based on the colony description and Gram or Spore stains.<LI> | ||
'''Isolation:'''<BR> | '''Isolation:'''<BR> | ||
