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BISC209: Enrichment: Difference between revisions
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→Enrichment media and Isolation protocols (Starting with either SOIL or SOIL EXTRACT)
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'''Modular Arginine-Glycerol-Salt Agar medium (AGS) medium for ''Streptomyces'' sp enrichment''' <BR> | '''Modular Arginine-Glycerol-Salt Agar medium (AGS) medium for ''Streptomyces'' sp enrichment''' <BR> | ||
Note that you will not be able to start this protocol until LAB3. Your instructors will heat dessicate (oven bake) your soil samples collected in LAB2 and return them to you in LAB3. You will use the heat shocked, dry soil sample to make a new soil extract for this protocol, which is based on spore resistance to dessication. Drying and heating the sample has encouraged spore generating bacteria to form a state that will allow them to survive harsh environmental conditions while killing off many of the microbes that can't make spores or survive the heat or lack of moisture. Now we need to coax those spores back into their vegetative state. The AGS medium uses cycloheximide to inhibit fungal growth since many fungi make spores, too. We hope to select for the ''Streptomyces'', a common and important genera of soil bacteria. This group is an important source of many antibiotics used to control human bacterial infections. Every soil isolate should be tested with this enrichment protocol. | Note that you will not be able to start this protocol until LAB3. Your instructors will heat dessicate (oven bake) your soil samples collected in LAB2 and return them to you in LAB3. You will use the heat shocked, dry soil sample to make a new soil extract for this protocol, which is based on spore resistance to dessication. Drying and heating the sample has encouraged spore generating bacteria to form a state that will allow them to survive harsh environmental conditions while killing off many of the microbes that can't make spores or survive the heat or lack of moisture. Now we need to coax those spores back into their vegetative state. The AGS medium uses cycloheximide to inhibit fungal growth since many fungi make spores, too. We hope to select for the ''Streptomyces'', a common and important genera of soil bacteria. This group is an important source of many antibiotics used to control human bacterial infections. Every soil isolate should be tested with this enrichment protocol. | ||
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'''Enrichment:'''<BR> | '''Enrichment:'''<BR> | ||
