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BISC209: Aseptic Transfer: Difference between revisions
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→Broth to Broth or Broth to Plate Transfer
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5. Insert the loop containing the culture into the destination tube of sterile broth, swirl gently and remove. If the destination is a plate of solid medium, follow the directions for streaking for isolation or for a spread plate found in the protocols section of the wiki. <br> | 5. Insert the loop containing the culture into the destination tube of sterile broth, swirl gently and remove. If the destination is a plate of solid medium, follow the directions for streaking for isolation or for a spread plate found in the protocols section of the wiki. <br> | ||
6. Replace the cover and set the newly inoculated broth tube in the rack. <br> | 6. Replace the cover and set the newly inoculated broth tube in the rack. <br> | ||
7. | 7. Re-sterilize the loop before putting it down by inserting the loop into the flame ''very slowly''! Doing this slowly allows any liquid remaining on the loop to evaporate rather than boil and avoid splattering live bacterial cells all over the bench and you.<br> | ||
=='''Use and Preparation of Stock Cultures on Slants'''== | =='''Use and Preparation of Stock Cultures on Slants'''== | ||
