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BioMicroCenter:Microarrays: Difference between revisions
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(New page: {{BioMicroCenter}} right|300px == Platforms == === Affymetrix === The MIT BioMicro Center preforms expression analysis using Affymetrix arrays. Our service inc...) |
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We encourage researchers to contact us several days in advance of sample submission to allow us lead time to purchase any specialized Affymetrix arrays. While we keep a few arrays in stock, most arrays are ordered on an as needed basis. A list of some of the available arrays and their prices can be found [[Media:Affy_Arrays_081218.pdf| in this file]]. Information about Affymetrix arrays is available at http://www.affymetrix.com/products_services/index.affx | We encourage researchers to contact us several days in advance of sample submission to allow us lead time to purchase any specialized Affymetrix arrays. While we keep a few arrays in stock, most arrays are ordered on an as needed basis. A list of some of the available arrays and their prices can be found [[Media:Affy_Arrays_081218.pdf| in this file]]. Information about Affymetrix arrays is available at http://www.affymetrix.com/products_services/index.affx | ||
All submissions must be accompanied by a [[BioMicroCenter:Forms#Downloads | Affymetrix Submission Form]]. | All submissions must be accompanied by a [[BioMicroCenter:Forms#Downloads|Affymetrix Submission Form]]. | ||
=== Affymetrix Sample Concentrations === | === Affymetrix Sample Concentrations === | ||
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Prokaryote 10 ug >0.6 ug/ul | Prokaryote 10 ug >0.6 ug/ul | ||
Euk. 3' Array 3 ug >0.3 ug/ul | Euk. 3' Array 3 ug >0.3 ug/ul | ||
[[3primeIVT|**New Kit**]] 0.1 ug http://www.affymetrix.com/products_services/reagents/specific/3_ivtexpresskit.affx | [[BioMicroCenter:3primeIVT|**New Kit**]] 0.1 ug http://www.affymetrix.com/products_services/reagents/specific/3_ivtexpresskit.affx | ||
Euk. Exon Array 2 ug >0.5 ug/ul | Euk. Exon Array 2 ug >0.5 ug/ul | ||
'''January 2009''' | '''January 2009''' | ||
** Affymetrix is retiring their old 3' Array kits in September. The [[3primeIVT|new kits]] are faster, require less starting material, and slightly less expensive (prices will be adjusted soon). Please indicate which type of kit you would like to use when submitting your sample. | ** Affymetrix is retiring their old 3' Array kits in September. The [[BioMicroCenter:3primeIVT|new kits]] are faster, require less starting material, and slightly less expensive (prices will be adjusted soon). Please indicate which type of kit you would like to use when submitting your sample. | ||
Information about our evaluation of the new kits is [[3primeIVT|HERE]] | Information about our evaluation of the new kits is [[BioMicroCenter:3primeIVT|HERE]] | ||
=== Non-MIT Users === | === Non-MIT Users === | ||
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Affymetrix is switching their labeling kits to a new kit called [http://www.affymetrix.com/products_services/reagents/specific/3_ivtexpresskit.affx 3'IVT Express]<br> | Affymetrix is switching their labeling kits to a new kit called [http://www.affymetrix.com/products_services/reagents/specific/3_ivtexpresskit.affx 3'IVT Express]<br> | ||
Information about the new kit can be found [[3primeIVT|HERE]] | Information about the new kit can be found [[BioMicroCenter:3primeIVT|HERE]] | ||
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Affymetrix processing prices '''do not''' include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples. | Affymetrix processing prices '''do not''' include the cost of the array. In order to be sure we have arrays on hand, we encourage users to contact us several days in advance of submitting samples. | ||
'''ITEM''' '''[[CoreDeps|CORE LAB]]''' '''MIT''' '''NON-MIT''' '''unit''' '''NOTES''' | '''ITEM''' '''[[BioMicroCenter:CoreDeps|CORE LAB]]''' '''MIT''' '''NON-MIT''' '''unit''' '''NOTES''' | ||
Affy Expression Processing | Affy Expression Processing | ||
Standard $260 $430 $645 per array *kit will be retired Sep.2009 | Standard $260 $430 $645 per array *kit will be retired Sep.2009 | ||
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After you have completed your scan, please make sure to sign the scanner record form, which is near the equipment and to remove your files from the computer. CD-Rs are available if needed. | After you have completed your scan, please make sure to sign the scanner record form, which is near the equipment and to remove your files from the computer. CD-Rs are available if needed. | ||
=== [[2100BioAnalyzer|AGILENT BIOANALYZER]] === | === [[BioMicroCenter:2100BioAnalyzer|AGILENT BIOANALYZER]] === | ||
'''ITEM''' '''[[CoreDeps|CORE LAB]]''' '''MIT''' '''NON-MIT''' '''unit''' '''NOTES''' | '''ITEM''' '''[[BioMicroCenter:CoreDeps|CORE LAB]]''' '''MIT''' '''NON-MIT''' '''unit''' '''NOTES''' | ||
RNA $10 $10 $15 per sample | RNA $10 $10 $15 per sample | ||
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