Main public logs
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Combined display of all available logs of OpenWetWare. You can narrow down the view by selecting a log type, the username (case-sensitive), or the affected page (also case-sensitive).
- 20:13, 28 April 2020 KirkLab talk contribs created page New Page (Created page with "==Overview== ==Protocol== ===Materials/Reagents=== ===Recipe===")
- 17:26, 27 April 2020 KirkLab talk contribs created page Quenching Buffer (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 17:01, 27 April 2020 KirkLab talk contribs created page BS3 (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 16:33, 27 April 2020 KirkLab talk contribs created page BS3 Conjugation Buffer (Created page with "BS3 Conjugation Buffer 1. Overview 2. Purpose 3. Recipe 20 mM Sodium Phosphate, 0.15M NaCl (pH 7-9)")
- 16:28, 27 April 2020 KirkLab talk contribs created page '''BS3 Conjugation Buffer''' (Created page with "'''BS3 Conjugation Buffer''' 1. Overview<br> 2. Purpose<br> 3. Recipe <br> 20 mM Sodium Phosphate, 0.15M NaCl (pH 7-9)")
- 22:26, 24 April 2020 KirkLab talk contribs created page Dynabeads Protein G (Created page with " <div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME'...")
- 22:14, 24 April 2020 KirkLab talk contribs created page General Protocols (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 22:08, 24 April 2020 KirkLab talk contribs created page Antibodies Kirk Lab (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 21:26, 24 April 2020 KirkLab talk contribs created page Mobile Phases (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 21:00, 24 April 2020 KirkLab talk contribs created page HPLC grade H2O (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 20:53, 24 April 2020 KirkLab talk contribs created page Coomassie Stained SDS-PAGE In-gel Trypsin Digestion Protocol (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 20:32, 24 April 2020 KirkLab talk contribs created page PCR Reaction Mix (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 20:26, 24 April 2020 KirkLab talk contribs created page Neutralization Reagent (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 20:24, 24 April 2020 KirkLab talk contribs created page Alkaline Lysis Reagent (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 19:53, 24 April 2020 KirkLab talk contribs created page Fetal Bovine Serum (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 19:39, 24 April 2020 KirkLab talk contribs created page ITS - X (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 19:33, 24 April 2020 KirkLab talk contribs created page Penicillin/streptomycin (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 19:31, 24 April 2020 KirkLab talk contribs created page Penicillin/streptomysin (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 19:09, 24 April 2020 KirkLab talk contribs created page Trypsin (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 18:59, 24 April 2020 KirkLab talk contribs created page Collagenase, type 2 (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 18:55, 24 April 2020 KirkLab talk contribs created page DMEM (Created page with " <div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME'...")
- 17:57, 24 April 2020 KirkLab talk contribs created page 9M Urea (Created page with "'''8M Urea - ''' {|class="wikitable" |- ! !1mL !2mL !3mL !5mL <div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab |...")
- 17:47, 24 April 2020 KirkLab talk contribs created page Standard relax buffer (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 17:25, 24 April 2020 KirkLab talk contribs created page Phosphatase inhibitor (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 17:22, 24 April 2020 KirkLab talk contribs created page Protease inhibitor (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 17:17, 24 April 2020 KirkLab talk contribs created page Activating Solution (Created page with " <div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME'...")
- 17:13, 24 April 2020 KirkLab talk contribs created page DTT (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 17:04, 24 April 2020 KirkLab talk contribs created page Permeabilization solution (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 16:49, 24 April 2020 KirkLab talk contribs created page Relaxing solution (Created page with " '''Relaxing solution''' #30 ml relax (in -20C #1 bottom shelf, thaw) #Add 30ul protease inhibitor #Add 30 ul DTT (in -20C # 1)")
- 00:04, 23 April 2020 KirkLab talk contribs created page General Buffers (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 23:53, 22 April 2020 KirkLab talk contribs created page General Sample Prep & Genotyping (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 23:34, 22 April 2020 KirkLab talk contribs created page Myocyte Isolation and assays (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 22:56, 22 April 2020 KirkLab talk contribs created page Myofilament (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 22:49, 22 April 2020 KirkLab talk contribs created page Whole Tissue (Created page with "Kirk Lab Protocol <br> =='''I. Whole Tissue from Frozen Tissue'''== 1)Add 1 mL of Lysis buffer per 100 mg of tissue to a glass homogenization vile <br> 2)Homogenize at...")
- 22:47, 22 April 2020 KirkLab talk contribs created page Western Blotting (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 22:19, 22 April 2020 KirkLab talk contribs created page Immunohistochemistry (Created page with "coming soon!")
- 22:16, 22 April 2020 KirkLab talk contribs created page Immunoprecipitation (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 22:07, 22 April 2020 KirkLab talk contribs created page Immunostanining (Created page with "==Immunostaining Cardiomyocytes== *From Frozen Tissue '''== {|cellspacing="10" cellpadding="3" style="background:#ffffff; width: 700px;" ==='''Materials/Solutions'''=== *...")
- 21:59, 22 April 2020 KirkLab talk contribs created page Histology (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 20:56, 22 April 2020 KirkLab talk contribs created page Cell Culture Rules (Created page with "=='''Tissue Culture Room Guidelines'''== ==='''Safe Laboratory Practices'''=== #Always wear gloves (change gloves when contaminated, and dispose of used gloves wit...")
- 18:48, 22 April 2020 KirkLab talk contribs created page Mycoplasma Protocol (Created page with " <div style="padding: 10px; background-color:gold; width: 950px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME...")
- 18:25, 22 April 2020 KirkLab talk contribs created page Rules and Guidelines (Created page with "==Cell Culture Rules== ==Mycoplasma Protocol==")
- 18:13, 22 April 2020 KirkLab talk contribs created page File:Mycoplasma protocol.pdf
- 18:13, 22 April 2020 KirkLab talk contribs uploaded File:Mycoplasma protocol.pdf
- 21:35, 21 April 2020 KirkLab talk contribs created page KH Buffer (Created page with " '''KH Buffer'''==<br> {|class="wikitable" |- !Chemicals !Working solution !10X solution |- | NaCl | 140mM --- 8.8g | 81.8g |- | KCl | 4.8mM --- 0.35g | 3.58g |- | MgSO4 | 1...")
- 21:29, 21 April 2020 KirkLab talk contribs created page Protocols Kirk Lab (Created page with "=='''KH Buffer'''==<br> {|class="wikitable" |- !Chemicals !Working solution !10X solution |- | NaCl | 140mM --- 8.8g | 81.8g |- | KCl | 4.8mM --- 0.35g | 3.58g |- | MgSO4 |...")
- 21:28, 21 April 2020 KirkLab talk contribs created page Neonatal Cardiomyocyte isolation (Created page with " <div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME'...")
- 20:57, 21 April 2020 KirkLab talk contribs created page Cell Culture (Created page with "====Cell culture Protocols==== ===Buffer recipes=== ===Neonatal Cardiomyocyte isolation===")
- 05:01, 21 April 2020 KirkLab talk contribs created page Myocyte Isolation (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")
- 05:00, 21 April 2020 KirkLab talk contribs created page Electrophoresis - Kirk Lab (Created page with "<div style="padding: 10px; background-color:gold; width: 1000px; text-align: center"> KirkLab | <font face="trebuchet ms" size=5 style="color:maroon > '''HOME''...")